Mouse Fibulin-1(FBLN1) ELISA kit

Code CSB-EL008452MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
fibulin 1
Alternative Names
Fbln1Fibulin-1 ELISA kit; FIBL-1 ELISA kit; Basement-membrane protein 90 ELISA kit; BM-90 ELISA kit
Abbreviation
FBLN1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 ng/mL-200 ng/mL
Sensitivity
0.78 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FBLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1000Average %95
Range %89-99
1:2000Average %95
Range %91-101
1:4000Average %94
Range %89-98
1:8000Average %98
Range %92-103
Recovery
The recovery of mouse FBLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9185-97
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2002.419 2.319 2.369 2.203
1001.701 1.601 1.651 1.485
501.022 1.002 1.012 0.846
250.664 0.654 0.659 0.493
12.50.448 0.425 0.437 0.271
6.250.375 0.365 0.370 0.204
3.120.287 0.281 0.284 0.118
00.168 0.164 0.166  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse FBLN1 ELISA Kit was designed for the quantitative measurement of Mouse FBLN1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

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 Q&A
Q:

 Does the kit contain human and/or animal by-products? What is the standard made of?

A:
Thanks for your inquiry!Standard is NS0-derived recombinant mouse MMP7 protein
Standard of kit CSB-EL008452MO is NS0-derived recombinant mouse FBLN1 protein
The kit does not contain any human and/or animal by-products.

Target Background

Function
(From Uniprot)
Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes.
Gene References into Functions
  1. Fbln1c plays a key role in regulating airway remodelling and inflammation in chronic asthma. PMID: 28862768
  2. The FBLN1/FGF8 interaction may also be involved in the survival of neural crest cell population during development. PMID: 27402846
  3. the findings indicate that Fbln1 normally suppresses proximal OFT EMT preventing proximal cushion hypercellularity and blood cell accumulation. PMID: 25575930
  4. Fbln1 is a new positive modulator of the formation of membranous bone and endochondral bone in the skull PMID: 25201465
  5. fibulin-1 regulates versican-dependent events in ventricular morphogenesis by promoting ADAMTS1 cleavage of versican leading to suppression of trabecular cardiomyocyte proliferation mediated by the ErbB2-Map kinase pathway. PMID: 22183742
  6. short arm region of laminin-5 gamma2 chain: structure, mechanism of processing and binding to heparin, nidogen-1, fibulin-1 and fibulin-2 PMID: 11733994
  7. structural and functional characterization of promoter PMID: 11829738
  8. binding of HPV E6 protein to fibulin-1 PMID: 12200142
  9. fibulin-1 is a new regulator of ADAMTS-1-mediated proteoglycan proteolysis and may play an important role in proteoglycan turnover in tissues where there is overlapping expression PMID: 16061471
  10. A murine placental overgrowth phenotype is associated with elevated Fbln1 transcript levels. PMID: 16338003
  11. Fbln1 is required for the directed migration and survival of cranial neueral crest cells contributing to the development of pharyngeal glands, craniofacial skeleton, cranial nerves, aortic arch arteries, cardiac outflow tract and cephalic blood vessels. PMID: 18538758

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Fibulin family
Tissue Specificity
Detected in most organs (brain, heart, lung, spleen, liver and kidney). Neurons are the predominant source of production in the brain. Not expressed significantly by astrocytes or microglia.
Database Links
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