Mouse Gastric triacylglycerol lipase(LIPF) ELISA kit

Code CSB-EL012976MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lipase, gastric
Alternative Names LipfGastric triacylglycerol lipase ELISA Kit; GL ELISA Kit; Gastric lipase ELISA Kit; EC ELISA Kit
Abbreviation LIPF
Uniprot No. Q9CPP7
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LIPF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 96
Range % 93-99
1:2 Average % 87
Range % 83-90
1:4 Average % 102
Range % 95-107
1:8 Average % 84
Range % 80-88
The recovery of mouse LIPF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 103 98-107
EDTA plasma (n=4) 86 83-89
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.563 2.601 2.582 2.476
10 1.928 2.000 1.964 1.858
5 1.306 1.268 1.287 1.181
2.5 0.803 0.831 0.817 0.711
1.25 0.488 0.491 0.490 0.384
0.625 0.325 0.334 0.330 0.224
0.312 0.224 0.214 0.219 0.113
0 0.105 0.107 0.106  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Subcellular Location Secreted
Protein Families AB hydrolase superfamily, Lipase family
Database Links

KEGG: mmu:67717

STRING: 10090.ENSMUSP00000025680

UniGene: Mm.329816


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