Mouse Growth/differentiation factor 5(GDF5) ELISA kit

Code CSB-EL009349MO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P43027
Lead Time 5-7 working days
Abbreviation GDF5
Protein Biological Process 1 Developmental Protein
Target Name growth differentiation factor 5
Alias BMP14, CDMP1, LAP4, OS5, SYNS2, OTTHUMP00000030755|cartilage-derived morphogenetic protein-1
Species Mus musculus (Mouse)
Protein Biological Process 3 Chondrogenesis
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 39.06 pg/mL-2500 pg/mL
Sensitivity 9.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GDF5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 92-104
1:2 Average % 92
Range % 88-95
1:4 Average % 86
Range % 80-91
1:8 Average % 89
Range % 83-98
Recovery
The recovery of mouse GDF5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 87-96
EDTA plasma (n=4) 101 94-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2500 2.550 2.644 2.597 2.467
1250 2.113 2.132 2.123 1.993
625 1.597 1.544 1.571 1.441
312.5 0.847 0.856 0.852 0.722
156.25 0.541 0.581 0.561 0.431
78.12 0.348 0.347 0.348 0.218
39.06 0.243 0.245 0.244 0.114
0 0.134 0.125 0.130  
References
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Function Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (By similarity). Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (By similarity). Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8
Involvement in disease Defects in Gdf5 are the cause of brachypodism (bp) which alters the length and numbers of bones in the limbs but spares the axial skeleton.
Subcellular Location Secreted, Cell membrane
Protein Families TGF-beta family
Database Links

KEGG: mmu:14563

STRING: 10090.ENSMUSP00000048079

UniGene: Mm.4744

Pathway Hippo signaling pathway
TGF-beta signaling pathway

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