Mouse Growth/differentiation factor 9(GDF9) ELISA kit

Instructions
Code CSB-EL009352MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name growth differentiation factor 9
Alternative Names Gdf9 ELISA Kit; Gdf-9 ELISA Kit; Growth/differentiation factor 9 ELISA Kit; GDF-9 ELISA Kit
Abbreviation GDF9
Uniprot No. Q07105
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GDF9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %86-93
1:2Average %99
Range %92-105
1:4Average %88
Range %87-94
1:8Average %89
Range %84-97
Recovery
The recovery of mouse GDF9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9388-99
EDTA plasma (n=4)10095-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.326 2.530 2.428 2.253
1001.377 1.390 1.384 1.209
500.818 0.835 0.827 0.652
250.487 0.521 0.504 0.329
12.50.334 0.350 0.342 0.167
6.250.298 0.276 0.287 0.112
3.120.218 0.212 0.215 0.040
00.170 0.180 0.175
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Required for ovarian folliculogenesis.
Gene References into Functions
  1. The arginine residue in the pre-helix loop of GDF9 homodimer may prevent the inhibition from its pro-domain or directly alter receptor binding, but this residue in GDF9 does not significantly affect the heterodimer activity during heterodimer formation. PMID: 25253739
  2. A novel role for GDF9 in causing reproductive defects and suppressing tumor initiation in the Inha(-/-) mouse model. PMID: 23446452
  3. Embryo vitrification has no effect on ovarian morphology or expression of GDF9. PMID: 23141291
  4. The results show that chronic unpredictable stress suppresses GDF9 expression. PMID: 22205697
  5. GDF9 may contribute to the variation observed in follicular development, ovulation rate, and fecundity between mammals PMID: 22234469
  6. Data indicate that GDF-9 is absent in follicles of obese anovulatory/PCOS ovaries; upon treatment by heterotopic transplantation of gonadal adipose tissue from lean mice, expression of GDF-9 and ovulation appear to resume. PMID: 21557698
  7. E2 and oocyte-derived paracrine factors GDF9 and bone morphogenetic protein 15 coordinate to promote the development of cumulus cells PMID: 21047911
  8. GDF9-stimulated mitogenic activity in granulosa and cumulus cells is mediated through ERK1/2 activity. PMID: 20736313
  9. Intraovarian excess androgen in polycystic ovary syndrome may result in follicular arrest by down-regulating growth and differentiation factor-9 expression. PMID: 20032061
  10. Knockout of pentraxin 3, a downstream target of growth differentiation factor-9, causes female subfertility. PMID: 12040004
  11. GNCF-dependent repression of Gdf9 mediates gamete regulation of female fertility. PMID: 12912906
  12. Growing oocytes and GDF9 can each induce granulosa cells from secondary follicles to shift their phenotype toward that of mural granulosa cells. PMID: 14695909
  13. GDF9 appears to function indirectly to regulate early granulosa cell proliferation and theca recruitment in vivo PMID: 15016837
  14. the endogenous GDF9 protein is an important oocyte-secreted mitogen, but accounts for only part of total oocyte bioactivity. PMID: 15128595
  15. gremlin modulates the cross-talk between GDF9 and BMP signaling that is necessary during follicle development PMID: 15133038
  16. GDF9 is a key mediator of oocyte-enabled cumulus expansion in mice. PMID: 15385414
  17. Gdf9 has a role in the development and function of the oocyte-cumulus cell complex in mice. PMID: 15531364
  18. neither GDF9 nor TGFbeta alone, nor the two in unison, account for the critical oocyte-secreted factors regulating mouse cumulus expansion PMID: 15761035
  19. The E-box is a key regulatory sequence for Gdf9 expression in the ovary. PMID: 16495478
  20. The GDF9 immunostaining was exclusively seen in growing oocytes PMID: 16943361
  21. NOBOX directly regulates the transcription of Pou5f1 and Gdf9 in oocytes during early folliculogenesis PMID: 16997917
  22. Exogenous GDF9 during in vitro maturation(IVM) improved embryo development and fetal viability and provides a promising approach for human IVM. PMID: 17933754
  23. This study is the first characterization of purified biologically active human GDF9 and as such is of importance for studies on human fertility. PMID: 18162287
  24. Its promoter, stably transfected into XX mouse ES cells, showed increased expression by addition of leukemia inhibitory factor to the media. PMID: 18593614
  25. studies confirm GDF9 expression in the mouse testis, pituitary gland and adrenocortical cancer (AC) cells, and establish its expression in L beta T2 gonadotrophs, and in mouse adrenal glands, particularly foetal and neonatal cortical cells PMID: 19505950
  26. The highest Gdf9 mRNA levels were detected in oocytes in the pre-antral follicle stage. PMID: 19553355

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Subcellular Location Secreted
Protein Families TGF-beta family
Tissue Specificity Ovary.
Database Links

KEGG: mmu:14566

STRING: 10090.ENSMUSP00000018382

UniGene: Mm.490461

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