Mouse Haptoglobin,Hpt/HP ELISA KIT

Instructions
Code CSB-E08586m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name haptoglobin
Alternative Names BP, HP2-ALPHA-2, HPA1S, MGC111141, binding peptide|haptoglobin alpha(1S)-beta|haptoglobin alpha(2FS)-beta|haptoglobin, alpha polypeptide|haptoglobin, beta polypeptide
Abbreviation HP
Uniprot No. Q61646
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 0.156 μg/mL-10 μg/mL
Sensitivity 0.039 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hpt in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 107
Range % 103-110
1:200 Average % 85
Range % 81-88
1:400 Average % 98
Range % 94-103
1:800 Average % 104
Range % 100-108
Recovery
The recovery of mouse Hpt spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 88 84-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average
10 0.103 0.105 0.104
5 0.169 0.163 0.166
2.5 0.249 0.251 0.250
1.25 0.388 0.398 0.393
0.625 0.629 0.612 0.621
0.312 0.919 0.908 0.914
0.156 1.245 1.251 1.248
0 1.874 1.901 1.888
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway (By similarity).
Subcellular Location Secreted
Protein Families Peptidase S1 family
Tissue Specificity Expressed by the liver and secreted in plasma.
Database Links

KEGG: mmu:15439

STRING: 10090.ENSMUSP00000074436

UniGene: Mm.26730

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