Product Details

Target Name

haptoglobin

Alternative Names

BP, HP2-ALPHA-2, HPA1S, MGC111141, binding peptide|haptoglobin alpha(1S)-beta|haptoglobin alpha(2FS)-beta|haptoglobin, alpha polypeptide|haptoglobin, beta polypeptide

Abbreviation

Uniprot No.

Q61646

Species

Mus musculus (Mouse)

Sample Types

serum, plasma

Detection Range

0.156 μg/mL-10 μg/mL

Sensitivity

0.039 μg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Competitive

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hpt in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	107
	Range %	103-110
1:200	Average %	85
	Range %	81-88
1:400	Average %	98
	Range %	94-103
1:800	Average %	104
	Range %	100-108

Recovery

The recovery of mouse Hpt spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	88	84-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
10	0.103	0.105	0.104
5	0.169	0.163	0.166
2.5	0.249	0.251	0.250
1.25	0.388	0.398	0.393
0.625	0.629	0.612	0.621
0.312	0.919	0.908	0.914
0.156	1.245	1.251	1.248
0	1.874	1.901	1.888

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Still Have Questions?

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Target Data

Function	As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway (By similarity).
Subcellular Location	Secreted
Protein Families	Peptidase S1 family
Tissue Specificity	Expressed by the liver and secreted in plasma.
Database Links	KEGG: mmu:15439 STRING: 10090.ENSMUSP00000074436 UniGene: Mm.26730

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Check Code：

Code	CSB-E08586m
Size	96T,5×96T,10×96T
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Mouse Haptoglobin,Hpt/HP ELISA KIT

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