Mouse High mobility group protein B2(HMGB2) ELISA kit

Code CSB-EL010560MO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P30681
Lead Time 7-14 working days
Abbreviation HMGB2
Target Name high-mobility group box 2
Alias HMG2, high-mobility group (nonhistone chromosomal) protein 2
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HMGB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 88-97
1:2 Average % 89
Range % 85-93
1:4 Average % 91
Range % 88-95
1:8 Average % 95
Range % 90-100
Recovery
The recovery of mouse HMGB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 85 80-90
EDTA plasma (n=4) 97 92-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.762 2.617 2.690 2.561
10 1.601 1.548 1.575 1.446
5 0.995 1.026 1.011 0.882
2.5 0.606 0.629 0.618 0.489
1.25 0.407 0.385 0.396 0.267
0.625 0.293 0.312 0.303 0.174
0.312 0.181 0.174 0.178 0.049
0 0.127 0.131 0.129  
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Function Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. In the nucleus is an abundant chromatin-associated non-histone protein involved in transcription, chromatin remodeling and V(D)J recombination and probably other processes. Binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters by enhancing transcription factor binding and/or bringing distant regulatory sequences into close proximity (By similarity). Involved in V(D)J recombination by acting as a cofactor of the RAG complex
Subcellular Location Nucleus, Chromosome, Cytoplasm, Secreted
Protein Families HMGB family
Tissue Specificity Widely expressed in embryo. In adult mainly expressed in lymphoid organs and testes (PubMed:11262228). Expressed in primary spermatocytes. Expressed in the superficial zone of articular cartilage (PubMed:19805379).
Database Links

KEGG: mmu:97165

STRING: 10090.ENSMUSP00000065940

UniGene: Mm.276881

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