Mouse Hyaluronidase-1(HYAL1) ELISA kit

Instructions
Code CSB-EL010918MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name hyaluronoglucosaminidase 1
Alternative Names Hyal1Hyaluronidase-1 ELISA Kit; Hyal-1 ELISA Kit; EC 3.2.1.35 ELISA Kit; Hyaluronoglucosaminidase-1 ELISA Kit
Abbreviation HYAL1
Uniprot No. Q91ZJ9
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 2.344 U/mL-150 U/mL
Sensitivity 0.586 U/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HYAL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 85
Range % 80-89
1:2 Average % 92
Range % 88-97
1:4 Average % 96
Range % 91-100
1:8 Average % 87
Range % 82-91
Recovery
The recovery of mouse HYAL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 85-94
EDTA plasma (n=4) 102 98-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/ml OD1 OD2 Average Corrected
150 2.114 2.268 2.191 1.988
75 1.563 1.514 1.539 1.336
37.5 1.072 1.109 1.091 0.888
18.75 0.729 0.776 0.753 0.550
9.375 0.538 0.522 0.530 0.327
4.688 0.361 0.341 0.351 0.148
2.344 0.256 0.263 0.260 0.057
0 0.201 0.205 0.203  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth.
Gene References into Functions
  1. It was concluded that: HYAL1 and HYAL2 are both needed for tissue HA catabolism; 2) HYAL2 is required for high MW HA clearance in lymph nodes and plasma and for HA endocytosis by liver NPCs; and 3) the main role of HYAL1 is HA degradation within liver NPCs. PMID: 26887442
  2. Findings suggest that HYAL1 contributes to endothelial and glycocalyx dysfunction induced by diabetes. HYAL1 inhibitors could be explored as a new therapeutic approach to prevent vascular complications in diabetes. PMID: 27246914
  3. HYAL1 is necessary for the breakdown of intracellular HA in the cortex, whereas HYAL2 is essential for the degradation of extracellular HA in all kidney regions PMID: 25468725
  4. The activity of the processed form of Hyal-1 was largely underestimated. PMID: 24502338
  5. The knockdown of Hyal-1 results in an 80% decrease of total acid hyaluronidase activity in the mouse liver, confirming that Hyal-1 is a key actor of hyaluronic acid catabolism in this organ. PMID: 24667601
  6. Chondroitin sulfate is a crucial determinant for skeletal muscle development/regeneration and improvement of muscular dystrophies through HYAL1 PMID: 23007393
  7. both HYAL1 and beta-hexosaminidase cleave chondroitin sulfate, but it is a preferred substrate for beta-hexosaminidase. These studies provide in vivo evidence to support and extend existing knowledge of GAG breakdown. PMID: 22451654
  8. Data show that Hyal-1, but not Hyal-3,is an ovarian regulator factor for follicle development, and show an interrelationship between this enzyme and the follistatin/activin/Smad3 pathway. PMID: 21732362
  9. Endothelial surface layer degradation by chronic hyaluronidase infusion induces proteinuria in apolipoprotein E-deficient mice PMID: 21170388
  10. Characterization of the murine hyaluronidase gene region reveals complex organization and cotranscription of Hyal1 with downstream genes, Fus2 and Hyal3. PMID: 11929860
  11. Hyal1 null mice had evidence of osteoarthritis with a loss of proteoglycans at early ages. PMID: 18344557
  12. Hyaluronidase induces ovarian granulosa cell apoptosis and is involved in follicular atresia. PMID: 18653706

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Subcellular Location Secreted, Lysosome
Protein Families Glycosyl hydrolase 56 family
Tissue Specificity Highly expressed in liver, kidney, lung and skin.
Database Links

KEGG: mmu:15586

STRING: 10090.ENSMUSP00000010195

UniGene: Mm.475658

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