Mouse Integrin alpha-L(ITGAL) ELISA kit

Code CSB-EL011875MO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P24063
Lead Time 5-7 working days
Abbreviation ITGAL
Protein Biological Process 1 Cell Adhesion
Target Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Alias CD11A, LFA-1, LFA1A, LFA-1 alpha|antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide|integrin alpha L|integrin gene promoter|lymphocyte function-associated antigen 1
Species Mus musculus (Mouse)
Protein Biological Process 3 Cell adhesion
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.44 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ITGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 96
Range % 92-100
1:2 Average % 88
Range % 84-92
1:4 Average % 103
Range % 99-107
1:8 Average % 94
Range % 90-98
Recovery
The recovery of mouse ITGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 85-94
EDTA plasma (n=4) 97 93-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.321 2.468 2.395 2.227
750 1.639 1.605 1.622 1.454
375 0.960 1.013 0.987 0.819
187.5 0.622 0.654 0.638 0.470
93.75 0.420 0.412 0.416 0.248
46.88 0.322 0.327 0.325 0.157
23.44 0.281 0.278 0.280 0.112
0 0.166 0.169 0.168  
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Function Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4
Subcellular Location Cell membrane, Single-pass type I membrane protein
Protein Families Integrin alpha chain family
Tissue Specificity Leukocytes.
Database Links

STRING: 10090.ENSMUSP00000101913

UniGene: Mm.1618

Pathway Regulation of actin cytoskeleton
Leukocyte transendothelial migration
Natural killer cell mediated cytotoxicity
Rap1 signaling pathway

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