Mouse Interferon β,IFN-β/IFNB ELISA Kit

Instructions
Code CSB-E04945m
Size 96T,5×96T,10×96T

Product Details

Target Name interferon, beta 1, fibroblast
Alternative Names IFB, IFF, IFNB, MGC96956, interferon beta
Abbreviation IFNB1
Uniprot No. P01575
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-β/IFNB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %90-103
1:2Average %89
Range %84-95
1:4Average %100
Range %95-106
1:8Average %97
Range %92-104
Recovery
The recovery of mouse IFN-β/IFNB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8993-96
EDTA plasma (n=4)10095-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.516 2.568 2.542 2.437
5002.115 2.135 2.125 2.020
2501.401 1.435 1.418 1.313
1250.761 0.730 0.746 0.641
62.50.343 0.369 0.356 0.251
31.20.242 0.253 0.248 0.143
15.60.179 0.172 0.176 0.071
00.104 0.106 0.105  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Q&A and Customer Reviews

 Customer Reviews
  • Sample type: cell culture supernatant or serum
    Sample species: Mouse
    Review: Extracellular stimulation of WRS elicits innate immune responses. (A) ELISA of IFN- (left) and IL-6 (right) levels in the supernatant of THP-1 cells treated with the indicated dose of rWRS for 12 or 24 h. LPS (100 ng) was used as a positive control. (B) ELISA of IFN- (upper left), IL-6 (upper right), IFN- (lower left), and TNF- (lower right) levels in the supernatant of Raw264.7 cells treated with the indicated dose of rWRS for 12 or 24 h. LPS (100 ng/ml) was used as a positive control.
    PMID: 30355684
  • Sample type: Serum and liver protein lysate
    Sample species: Mouse
    Review: ELISA analysis of serum IFN-b levels. Data are expressed as the mean ± SD from at least three independent experiments (n=5).
    PMID: 30448255

Target Data

Function Has antiviral, antibacterial and anticancer activities.
Subcellular Location Secreted
Protein Families Alpha/beta interferon family
Database Links

KEGG: mmu:15977

STRING: 10090.ENSMUSP00000056720

UniGene: Mm.1245

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