Mouse Interferon β,IFN-β/IFNB ELISA Kit

Instructions
Code CSB-E04945m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interferon, beta 1, fibroblast
Alternative Names Ifnb1 ELISA Kit; Ifb ELISA Kit; IfnbInterferon beta ELISA Kit; IFN-beta ELISA Kit
Abbreviation IFNB1
Uniprot No. P01575
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-β/IFNB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %90-103
1:2Average %89
Range %84-95
1:4Average %100
Range %95-106
1:8Average %97
Range %92-104
Recovery
The recovery of mouse IFN-β/IFNB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8993-96
EDTA plasma (n=4)10095-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.516 2.568 2.542 2.437
5002.115 2.135 2.125 2.020
2501.401 1.435 1.418 1.313
1250.761 0.730 0.746 0.641
62.50.343 0.369 0.356 0.251
31.20.242 0.253 0.248 0.143
15.60.179 0.172 0.176 0.071
00.104 0.106 0.105  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

 Customer Reviews
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Sample type: Biological fluids(liver protein lysate)

Sample species: Mouse

Review: ELISA analysis of serum IFN-b levels. Data are expressed as the mean ± SD from at least three independent experiments (n=5).

By Anonymous

Sample type: Cell culture supernatant

Sample species: Mouse

Review: Extracellular stimulation of WRS elicits innate immune responses. (A) ELISA of IFN- (left) and IL-6 (right) levels in the supernatant of THP-1 cells treated with the indicated dose of rWRS for 12 or 24 h. LPS (100 ng) was used as a positive control. (B) ELISA of IFN- (upper left), IL-6 (upper right), IFN- (lower left), and TNF- (lower right) levels in the supernatant of Raw264.7 cells treated with the indicated dose of rWRS for 12 or 24 h. LPS (100 ng/ml) was used as a positive control.

By Anonymous

Target Background

Function
(From Uniprot)
Has antiviral, antibacterial and anticancer activities.
Gene References into Functions
  1. IFNbeta activates neuronal PI3K/Akt signalling and Akt binds to transcription factor FoxA1 that translocates to the nucleus and induces PDL1. Conversely, inhibition of PI3K/Akt, FoxA1 and PDL1 blocked neuronal ability to generate FoxA1(+)Tregs. PMID: 28436428
  2. Macrophages activated by metabolic endotoxemia infiltrated into islets and produced IFNbeta, which induced beta-cell apoptosis by increasing the expression of Xaf1. PMID: 29132171
  3. Reactive oxygen species (ROS) scavenger N-acetyl cysteine (NAC) prevented mitochondrial dysfunctions, type I IFN-stimulated transcript levels, inflammatory cell infiltrate, and muscle weakness in an experimental autoimmune myositis mouse model. Thus, these data highlight a central role of mitochondria and ROS in dermatomyositis . PMID: 28623559
  4. Nontypeable Haemophilus influenzae DNA as a Pathogen-Associated Molecular Pattern Molecules triggered I-IFN response, which was STING/TBK1/IRF3 dependent. PMID: 29421524
  5. The Lipopolysaccharide and IFN-beta-mediated increase of STAT1 mRNA and protein levels was abrogated by chelation of Zn(2+) with the membrane permeable chelator N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) in RAW 264.7 macrophages. PMID: 28965604
  6. MAVS is essential for spontaneous high basal expression of IFN-beta in cardiac myocytes and the heart. PMID: 28822807
  7. Mycobacterium smegmatis induces higher Ifnb expression in macrophages than Mycobacterium avium subspecies. PMID: 28422568
  8. Chronic presence of IFN-I in the brain microenvironment, which negatively affects cognitive function, is mediated via modulation of microglial activity. PMID: 28959042
  9. In experimental autoimmune encephalomyelitis, IFN-beta inhibited downstream inflammatory cytokines through the inhibition of PI3K/AKT/NF-kappaB axis and p38, JNK-MAPK, as well as the regulation of mTOR complexes. Moreover, IFN-beta inhibited Th17 differentiation and influenced the acetylation of the Il17a and Opn gene promoters. IFN-beta plays a role in Th17 differentiation partly through the inhibition of OPN. PMID: 29127843
  10. This review briefly discusses the dysregulation of main T cell subpopulations in CNS autoimmunity and summarized the T cell targeted effects of endogenous and exogenous IFN-beta in health and EAE/MS, with emphasis on the direct actions of IFN-beta on each T cell subset involved in the disease. PMID: 27033173
  11. Rb selectively inhibits innate IFN-beta production by enhancing deacetylation of Ifnb1 promoter, exhibiting a previous unknown non-classical role in innate immunity, which also suggests a role of Rb in the regulation of type I IFN production in inflammatory or autoimmune diseases. PMID: 27267461
  12. The effect of topical TREX1 knockdown and local interferon production on HIV transmission in human cervicovaginal explants and humanized mice, is reported. PMID: 27184854
  13. The TBK1 Y179A mutant failed to rescue type I IFN production by virally infected RAW264.7 macrophages deficient in TBK1. PMID: 28049762
  14. The data demonstrate that an atypical TLR7 signaling pathway contributes to type interferon-beta expression during Y. pestis infection and suggest that the TLR7-driven type I IFN response plays an important role in determining the outcome of plague. PMID: 28847850
  15. Long-term exposure to atmospheric particulates, PM2.5 up-regulated H3K4 and H3K9 methylation in IL-6 and IFN-beta promoter regions through down-regulating Kdm6a expression. The results suggest that short-term exposure to PM2.5 significantly enhances the survival rate of influenza A-contaminated mice, while long-term PM2.5 inhalation lowers the capacity of pulmonary macrophages to secrete IL-6 and IFN-beta. PMID: 28887033
  16. Extracellular ATP reduces the replication of VSV, Newcastle disease virus, murine leukemia virus and HSV in vivo and in vitro through the P2X7 receptor; ATP increases IFN-beta expression. Mechanistically, ATP facilitates IFN-beta secretion through P38/JNK/ATF-2 signaling pathways, which are crucial in promoting antiviral immunity. PMID: 28687662
  17. study reports that mitochondrial antiviral signaling protein (MAVS), -mediated IFN-alpha/beta production and IFNAR signaling are required for alloimmune responses to the human K1 Ag in a murine model of inflammation-induced alloimmunization PMID: 28630094
  18. study identifies a novel role for RIPK1 and RIPK3, a pair of homologous serine/threonine kinases previously implicated in the regulation of necroptosis and pathologic tissue injury, in directing IFN-beta production in macrophages PMID: 28461567
  19. results suggest that, in addition to its well-known signaling activity, IFN-beta may be directly antimicrobial and be part of a growing family of cytokines and chemokines, called kinocidins, that also have antimicrobial properties. PMID: 28411186
  20. results show that resistance to HSV-1 in the trigeminal ganglia during acute infection is conferred in part by STING and IFN-alpha/beta signaling in both bone marrow-derived and -resident cells, which coalesce to support a robust HSV-1-specific CD8(+) T cell response PMID: 27511736
  21. RIPK3 regulates type I IFN both transcriptionally, by interacting with MAVS and limiting RIPK1 interaction with MAVS, and post-transcriptionally. PMID: 28410401
  22. neddylation contributes to HSV-1-induced early phase IFN-beta production. PMID: 27593482
  23. Rubicon specifically interacts with the interferon regulatory factor (IRF) association domain (IAD) of IRF3, and this interaction leads to inhibition of the dimerization of IRF3, which negatively regulates interferon-mediated antiviral response. PMID: 28468885
  24. Translation arrest is further demonstrated to be key for anti-viral response by acting synergistically with MAVS activation to amplify TBK1 signaling and IFN-beta mRNA transcription, while GADD34-dependent protein synthesis recovery contributes to the heterogeneous expression of interferon observed in dsRNA-activated cells. PMID: 28100675
  25. The authors report that Raf kinase inhibitory protein (RKIP) is essential for TBK1 activation and type I interferon production triggered by viral infection. PMID: 27753621
  26. this study shows that paracrine type I IFN can profoundly enhance innate recognition and TLR2-dependent transcriptional responses to Francisella tularensis infection upstream of its role in inflammasome regulation in primary murine peritoneal macrophages but not murine bone marrow-derived macrophages PMID: 27231145
  27. STAT2 recruits USP18 to the type I IFN receptor subunit IFNAR2 via its constitutive membrane-distal STAT2-binding site. PMID: 28165510
  28. In the exorbital lachrymal gland, the mRNA expression of IFN beta and IFN alpha (type I IFNs) was weak- to strong-positive at 1 days post inoculation (DPI), and became negative at 2DPI. PMID: 27079771
  29. TBK1 complexes required for the phosphorylation of IRF3 and the production of interferon-beta have been identified. PMID: 28159912
  30. The authors conclude that IFN-alphabeta is pathogenic during chronic Mycobacterium africanum infection and that the pathogenic effects may be mediated through poorer control of bacterial growth. PMID: 27803172
  31. IRF-1 and interferon-alpha with interferon-beta cooperate to control acute gammaherpesvirus infection. PMID: 27795415
  32. this study shows that endosomal signalling by Lactobacillus acidophilus that leads to IFN-beta production is inhibited by TLR stimulation from the plasma membrane PMID: 27441725
  33. Truncated rabies virus phosphoprotein isoforms promote viral replication in muscle cells through their IFN-beta antagonist activities and thereby support infection of peripheral nerves. PMID: 27384657
  34. identify iNOS/NO as an integral component of IFN-beta production in response to dsRNA in hepatocytes in a pathway that involves the coordinated activities of TLR3/Trif and PKR PMID: 27226571
  35. Data show that using adipose-derived mesenchymal stem cells (AD-MSCs) expressing interferon-beta (IFN-beta) as an anti-inflammatory agent, offer evidence supporting that the stem cell therapies in experimental autoimmune encephalomyelitis (EAE) may improve the valuable effects of IFN-beta in the autoimmune disease. PMID: 27373971
  36. NLRX1 was observed to reduce interferon beta and cytokines in response to HIV-1 reverse-transcribed DNA. PMID: 27078069
  37. This study establishes IL-1beta and IFN-I levels as key homeostatic variables of protective, yet tuned, immune responses against severe invasive bacterial infection. PMID: 26962946
  38. Type I IFN Does Not Promote Susceptibility to Foodborne Listeria monocytogenes PMID: 26895837
  39. Results suggest that the lack of autocrine response to interferon-betaI by the host cell may be one mechanism for maintenance of respiratory syncytial virus persistence. PMID: 26501312
  40. TRIM33 deficiency results in high expression of Ifnb1 at late stages of macrophages activation. PMID: 26592194
  41. Inhibition of PI3K or Akt abrogated the ability of Syk deficiency to enhance IFNbeta and IL-10 in Syk deficient cells PMID: 26708990
  42. IFN-beta significantly attenuated the progression of liver fibrosis, with accompanying transcriptional downregulation of the TLR4 adaptor molecule MyD88. PMID: 25715168
  43. Thus, despite their use of the same receptor, IFNbeta and IFNalpha have unique and distinguishable biologic functions, with IFNbeta being mainly responsible for promoting lymphocytic choriomeningitis virus persistence. PMID: 25974304
  44. Mice lacking Ifnb function exhibited motor and cognitive learning impairments with accompanying alpha-synuclein-containing Lewy bodies in the brain, as well as a reduction in dopaminergic neurons and defective dopamine signaling in the nigrostriatal region. PMID: 26451483
  45. Data show that interferon beta knockout IFN-beta(-/-) mice exhibit greater resistance to Salmonella typhimurium infection. PMID: 26202980
  46. results indicate that Lyn plays a positive regulatory role in RIG-I-mediated interferon expression as a downstream component of IPS-1 PMID: 25585356
  47. Data show that decreased type I interferon gene expression in T cell subsets of human CASP8 and FADD-like apoptosis regulating protein c-FLIPS transgenic female mice. PMID: 24816846
  48. Data suggest that Brd4 (bromodomain containing 4) is essential for Toll-like receptor-stimulated interferon-beta (Ifnb) gene transcription by permitting transcription factors to interact with the Ifnb promoter in macrophages. PMID: 25891802
  49. PP1 directly interacts with IRF3 and dephosphorylates IRF3 at Ser385 and Ser396, resulting in the suppression of TLR- and RLR-triggered IFN-beta production. PMID: 25239187
  50. Interferons alpha and beta control propagation of long interspersed element-1. PMID: 25716322

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Subcellular Location Secreted.
Protein Families Alpha/beta interferon family
Database Links

KEGG: mmu:15977

STRING: 10090.ENSMUSP00000056720

UniGene: Mm.1245

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