| Description |
This ELISA kit is used for quantitative detection of mouse IFN-γ, establishing normal detection range and validation of antibody array results. It can measure IFN-γ levels in various biological fluids, including serum, plasma, tissue homogenates, or cell culture supernates. IFN-γ is a pleiotropic molecule that exerts cytostatic, antiproliferative, pro-apoptotic, and antitumor effects. Studies also showed that IFN-γ may also stimulate pro-tumorigenic transformations and promote tumor progression. Its pleiotropic functions are mediated by cell-specific expression of hundreds of IFN-γ-regulated genes that encompass inflammatory signaling molecules, apoptosis and cell cycle regulators, and transcriptional activators.
Based on the Sandwich-ELISA principle and enzyme-substrate chromogenic reaction property, the users can measure the content of mouse IFN-γ in serum, plasma, tissue homogenates, or cell culture supernates. This mouse IFN-γ ELISA kit is quality tested with high specificity, excellent sensitivity, precision less than 10%, good linearity, and high recovery. Click the product instructions for more validation information. |
| Target Name |
interferon, gamma |
| Alternative Names |
Ifng ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit |
| Abbreviation |
IFNG |
| Uniprot No. |
P01580 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates, cell culture supernates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology
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| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
82-99 |
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| 1:2 |
Average % |
97 |
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| Range % |
87-105 |
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| 1:4 |
Average % |
95 |
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| Range % |
89-100 |
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| 1:8 |
Average % |
90 |
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| Range % |
82-98 |
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| Recovery |
| The recovery of mouse IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
85-93 |
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| EDTA plasma (n=4) |
99 |
92-107 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.889 |
2.785 |
2.837 |
2.670 |
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| 500 |
2.356 |
2.324 |
2.340 |
2.173 |
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| 250 |
1.571 |
1.614 |
1.593 |
1.426 |
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| 125 |
0.885 |
0.862 |
0.874 |
0.707 |
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| 62.5 |
0.569 |
0.542 |
0.556 |
0.389 |
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| 31.2 |
0.315 |
0.330 |
0.323 |
0.156 |
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| 15.6 |
0.254 |
0.247 |
0.251 |
0.084 |
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| 0 |
0.164 |
0.169 |
0.167 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse IFN-γ This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- Biotin-labeled IFN-γ antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
- HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- Biotin-antibody Diluent 1 x 15 ml ---Dilute the Biotin-antibody solution.
- HRP-avidin Diluent 1 x 15 ml ---Dilute the HRP-avidin solution.
- Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
- Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
- TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
