Mouse Kallikrein-1(KLK1) ELISA kit

Instructions
Code CSB-EL012446MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name kallikrein 1
Alternative Names Klk1 ELISA Kit; Klk-6 ELISA Kit; Klk6Kallikrein-1 ELISA Kit; EC 3.4.21.35 ELISA Kit; Glandular kallikrein K1 ELISA Kit; KAL-B ELISA Kit; Renal kallikrein ELISA Kit; Tissue kallikrein-6 ELISA Kit; mGK-6 ELISA Kit
Abbreviation KLK1
Uniprot No. P15947
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse KLK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:5 Average % 84
Range % 81-89
1:10 Average % 86
Range % 83-89
1:20 Average % 95
Range % 90-100
1:40 Average % 88
Range % 84-93
Recovery
The recovery of mouse KLK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 109 105-113
EDTA plasma (n=4) 87 83-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
5000 2.509 2.512 2.511 2.398
2500 1.724 1.704 1.714 1.601
1250 0.978 0.934 0.956 0.843
625 0.567 0.600 0.584 0.471
312 0.382 0.376 0.379 0.266
156 0.248 0.241 0.245 0.132
78 0.165 0.167 0.166 0.053
0 0.112 0.114 0.113  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Gene References into Functions
  1. Polypeptides identified based on immunorecognition by autoantibodies in sera from IQI/Jic mice affected with autoimmune disease (>12 weeks of age) were tissue kallikrein (Klk)-1 and -13 and were cross-reactive to the autoantibodies. PMID: 15545270
  2. Protective effects of Tissue kallikrein gene transfer on ischemic diseases. PMID: 17465807
  3. TK, like several other proteases, can activate ENaC in the kidney and the colon. PMID: 18086683
  4. Tissue kallikrein and kinins do not influence the trophicity of kidneys, the synthesis and secretion of renin, blood pressure increase, and cardiac remodeling due to renin angiotensin system activation. PMID: 19244588
  5. tissue kallikrein knockout mice had a significant two-fold increase in albuminuria PMID: 19516248
  6. The study suggests that kallikrein plays an antihypertensive role in hyperaldosteronism. PMID: 22669897
  7. These data do not support a role for the TK-kinin system, protective or deleterious, in the development of insulin resistance and diabetes. PMID: 24599937
  8. Lower excretion of active renin and prorenin in TK(-/-) compared to TK(+/+) suggest coordinated regulation of the two proteins in their participation to collecting duct function. PMID: 14633138
  9. Plasma kallikrein-deficient mice challenged with transient middle cerebral artery occlusion developed significantly smaller brain infarctions. PMID: 25628066
  10. Local expression of kallikreins within the kidney has the capacity to dampen lupus nephritis, possibly by modulating inflammation and oxidative stress. PMID: 23280471
  11. The data of this study presented support the conclusion that Klk6 is an important participant in the immunopathogenic events that drive TMEV-IDD at acute through early chronic time points. PMID: 22335454
  12. Tissue kallikrein is essential for invasive capacity of circulating proangiogenic cells. PMID: 21164105
  13. kallikrein 6 is expressed in the nonmalignant and malignant prostate, with cancer tissues demonstrating slightly lower expression PMID: 12970725
  14. Kallikrein 6 participates in enzymatic cascades mediating CNS inflammatory disease. PMID: 15033932
  15. Tissue-specific expression patterns and differential regulation in CNS disease indicates that each K6 5'-transcript is probably regulated by unique promoter elements and may serve as a molecular target to treat inflammatory demyelinating disease. PMID: 15584920
  16. Data suggest that tissue kallikrein 1 may be implicated in the growth of uterine endometrial tissues during the proliferative phase. PMID: 17219431
  17. These results suggest that LPS activates the kallikrein-kinin system in the choroid plexus via autocrine induction of IL-1beta and TNF-alpha. PMID: 18329172
  18. role of KLK1 in arterial function; role of KLK1 in the control of ionic transport in the renal tubule; cardio- and nephro-protective effects of KLK1 and kinins in acute cardiac ischemia, post-ischemic heart failure, and diabetes [review] PMID: 18627303
  19. KLK6, but not KLK1 is an activator of CNS protease-activated receptors PMID: 18778305
  20. Gene-transfer of wild-type Ad.hKLK1 (kallikrein 1) was able to rescue impaired muscle neovascularization in hindlimb ischemia in knockout mice PMID: 19164804
  21. These findings indicate that kallikreins play an important protective role in autoantibody-initiated glomerulonephritis (GN) and could constitute potential candidate genes for anti-glomerular basement membrane-induced GN and spontaneous lupus nephritis. PMID: 19262577

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Protein Families Peptidase S1 family, Kallikrein subfamily
Database Links

KEGG: mmu:16612

STRING: 10090.ENSMUSP00000074659

UniGene: Mm.142722

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