Mouse L-amino-acid oxidase(IL4I1) ELISA kit

Instructions
Code CSB-EL011660MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interleukin 4 induced 1
Alternative Names Il4i1 ELISA Kit; Fig1 ELISA Kit; L-amino-acid oxidase ELISA Kit; LAAO ELISA Kit; LAO ELISA Kit; EC 1.4.3.2 ELISA Kit; Interleukin-4-induced protein 1 ELISA Kit; IL4-induced protein 1 ELISA Kit; Protein Fig-1 ELISA Kit; mFIG1 ELISA Kit
Abbreviation IL4I1
Uniprot No. O09046
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL4I1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 101    
Range % 92-105    
1:2 Average % 94    
Range % 90-99    
1:4 Average % 95    
Range % 87-98    
1:8 Average % 92    
Range % 85-95    
Recovery
The recovery of mouse IL4I1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 103 94-107    
EDTA plasma (n=4) 98 90-102    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
1000 1.780 1.814 1.797 1.596    
500 1.316 1.301 1.309 1.108    
250 0.954 0.939 0.947 0.746    
125 0.716 0.732 0.724 0.523    
62.5 0.488 0.498 0.493 0.292    
31.2 0.406 0.400 0.403 0.202    
15.6 0.306 0.294 0.300 0.099    
0 0.203 0.199 0.201      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Lysosomal L-amino-acid oxidase with highest specific activity with phenylalanine. May play a role in lysosomal antigen processing and presentation.
Gene References into Functions
  1. Data showed proteome changes in alveolar epithelial cells type II exposed to an infection with A. fumigatus with IL4I1 showing the most prominent increase in abundance both, in terms of relative abundance as well as activity. Also, in the infected lungs, there was levels of IL4I1 metabolic products. PMID: 28951444
Subcellular Location Isoform 1: Lysosome, SUBCELLULAR LOCATION: Isoform 2: Lysosome
Protein Families Flavin monoamine oxidase family, FIG1 subfamily
Tissue Specificity Isoform 1 primarily found in immune tissues, mostly in B-lymphocytes. Isoform 2 restricted to the testis, predominantly in Sertoli cells at the periphery of the ducts, and the brain, including Purkinje cells, hippocampus and mitral cells in the olfactory
Database Links

KEGG: mmu:100328588

STRING: 10090.ENSMUSP00000113726

UniGene: Mm.2565

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