Mouse Leucine-rich repeat-containing G-protein coupled receptor 5(LGR5) ELISA kit

Instructions
Code CSB-EL012906MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name leucine-rich repeat-containing G protein-coupled receptor 5
Alternative Names Lgr5 ELISA kit; Fex ELISA kit; Gpr49Leucine-rich repeat-containing G-protein coupled receptor 5 ELISA kit; G-protein coupled receptor 49 ELISA kit; Orphan G-protein coupled receptor FEX ELISA kit
Abbreviation LGR5
Uniprot No. Q9Z1P4
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LGR5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 87-98
1:2 Average % 97
Range % 93-102
1:4 Average % 84
Range % 81-89
1:8 Average % 88
Range % 83-95
Recovery
The recovery of mouse LGR5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 98-104
EDTA plasma (n=4) 92 89-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.527 2.588 2.558 2.409
1000 2.166 2.101 2.134 1.985
500 1.634 1.682 1.658 1.509
250 1.125 1.130 1.128 0.979
125 0.564 0.560 0.562 0.413
62.5 0.351 0.344 0.348 0.199
31.25 0.220 0.212 0.216 0.067
0 0.148 0.149 0.149  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Gene References into Functions
  1. LGR5 promotes cancer stem cell traits and chemoresistance in cervical cancer. PMID: 28880275
  2. Results show that Lgr5 is expressed at the neuromuscular junction (NMJ) and is a receptor for Rspo2. PMID: 27328992
  3. Mex3a defines a reserve-like intestinal stem cell population within the Lgr5+ compartment. PMID: 28285904
  4. sorted Reg4(+) DCS cells promote organoid formation of single Lgr5(+) colon stem cells. DCS cells can be massively produced from Lgr5(+) colon stem cells in vitro by combined Notch inhibition and Wnt activation PMID: 27573849
  5. The Lgr5-expressing cells differentiate into hair cells in high yield. PMID: 28228258
  6. Single-cell transcript profiles reveal multilineage priming in early progenitors derived from Lgr5-expressing intestinal stem cells. PMID: 27524622
  7. Arrb1 reduced the chemotherapy-induced Lgr5 stem cell apoptosis by inhibiting endoplasmic reticulum stress-mediated mitochondrial apoptotic signaling. PMID: 27195676
  8. demonstrates that Lgr5 cells give rise to both anterior and posterior taste buds during development, and are capable of regenerating posterior taste buds after injury induced by glossopharyngeal nerve transection PMID: 23824276
  9. Lgr5(+) chief cells are involved in maintaining the homeostatic stem cell pool. Define Lgr5(+) chief cells as a major cell-of-origin of gastric cancer. PMID: 28581476
  10. Stem cell plasticity enables hair regeneration following Lgr5(+) cell loss. PMID: 28553937
  11. role for Lgr5(+) cancer stem cells in primary and metastatic colon cancer PMID: 28358093
  12. Data show that both the number and relative position of Paneth cells and Lgr5+ cells are important for fission. PMID: 27348469
  13. RSPO-LGR4/5-ZNRF3/RNF43 module controls metabolic liver zonation and is a hepatic growth/size rheostat during development, homeostasis and regeneration. PMID: 27088858
  14. adult stem cell marker leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5) is expressed in the mice nasal respiratory epithelium. PMID: 27773817
  15. Employing Lgr5-lacZ transgenic mice and Lgr5 in situ hybridization, authors found colonic epithelial stem cells (CESC) markedly more radioresistant in vivo than small intestinal crypt base columnar stem cells. PMID: 28202528
  16. Our studies show for the first time that Lgr5 is expressed in the NC cells at embryonic day 9.5 (E9.5) and is maintained during embryonic development and postnataly in the stroma of the ventral tongue, and some areas of the oral mucosa and that Lgr5+ cells participate in the maintenance of the stroma. PMID: 26865184
  17. Genetic fate-mapping of Alkaline phosphate intestinal(+) cells before or during targeted ablation of Lgr5-expressing stem cells generated numerous long-lived crypt-villus "ribbons," indicative of dedifferentiation of enterocyte precursors into Lgr5(+) stems. PMID: 26831517
  18. Report targeting LGR5+ tumor stem cells with an antibody-drug conjugate as effective treatment for colon cancer. PMID: 26582901
  19. The generation of new retinal cells, including retinal neurons and Muller glia from Lgr5-expressed amacrine cells, begins in early adulthood and continues as the animal ages. PMID: 25990970
  20. Myh9 mediates colitis-induced epithelium injury by restricting Lgr5+ stem cells PMID: 25968904
  21. H pylori colonize and manipulate the progenitor and Lgr5(+)stem cell compartments, which alters turnover kinetics and glandular hyperplasia. PMID: 25725293
  22. These data suggest that the neonatal mammalian cochlea has some capacity for hair cell regeneration following damage alone and that Lgr5-positive cells act as hair cell progenitors in the cochlea. PMID: 24672754
  23. Positioning within the niche and levels of LGR5 expression discriminate intestinal intestinal stem and progenitor cells with different Wnt/beta-catenin signaling activity. PMID: 25609789
  24. Organoids that continue to express Lgr5 are multipotent.all three Lgr transcripts Lgr4-Lgr6 were detected in sorted Lgr5+ cells from posterior tongue and in sorted Lgr6+ cells from either posterior or anterior tongue. PMID: 25368147
  25. The members of the R-spondin family are known as activators of Wnt signaling, and Lgr4, Lgr5, and Lgr6 have been identified as receptors for R-spondins. PMID: 24616052
  26. Using in vivo lineage tracing, identifed embryonic and neonate Lgr5(+) populations as stem/progenitor cells contributing to the development of the ovary surface epithelium cell lineage, as well as epithelia of the mesovarian ligament and oviduct/fimbria. PMID: 24997521
  27. LGR5 is required for correct timing of germ cell differentiation and is found in cells that give rise to adult cortical granulosa cells. PMID: 25158167
  28. Lgr5(+) cells are seen in a large number of gastric tumors ; their frequent basal arrangements and coexpression of ISC markers support the idea that Lgr5(+) cells act as stem cells during the early stage of intestinal-type gastric tumorigenesis. PMID: 24340024
  29. This report that Lgr5, a recently discovered adult stem cell marker, is exclusively expressed in GBCs in neonatal and adult mice PMID: 24920630
  30. Lgr4KO and Lgr4/5KO results in complete loss of intestinal stem cells in embryos, impairs proliferation of developing kidney and skin epithelium, and depletes Lgr5+follicular epithelium and impairs hair follicle development. PMID: 24680895
  31. LGR5 is critical for corpora lutea function and successful pregnancy in mice PMID: 24469993
  32. Lgr5 can be considered a universal SSC marker. PMID: 23475985
  33. Wnt/beta-catenin can drive Lgr5-positive cells to act as hair cell progenitors, even after their exit from the cell cycle and apparent establishment of cell fate. PMID: 23918377
  34. The results suggested that CD61 signaling is required for the proper growth of the cervical loop and for the promotion of the proliferation of pre-ameloblastic cells through Lgr5. PMID: 23553959
  35. large subset of the Lgr5(+) stem cell progeny, expressing K15 and P-cadherin, is aberrantly mobilized to the upper region of hair follicles and the epidermis PMID: 22945646
  36. In SACK-expanded hair follicle stem cells, nuclear Lgr5 is expressed in both sisters during symmetric self-renewal divisions, which are associated with random chromosome segregation. PMID: 22355691
  37. TROY is up-regulated in human colorectal cancer cell lines and in intestinal tumors in mice. It functions as a negative modulator of the Wnt pathway in LGR5-positive stem cells. PMID: 23142137
  38. The outstanding regenerative potential of a rare population of Lgr5-expressing (Lgr5(+)) mammary epithelial cells. PMID: 23352663
  39. The expression of Lgr5 protein is not a prerequisite for proper thymus organogenesis. PMID: 23423968
  40. Stem cell marker Lgr5 protein was found in clusters of epithelial cells of periodontal ligament in aging mice, suggesting a maintenance role for epithelial stem cells throughout the life cycle. PMID: 23167464
  41. Lgr5 is not expressed in healthy adult liver; however, small Lgr5(+) cells appear near bile ducts upon liver damage, coinciding with robust activation of Wnt signalling PMID: 23354049
  42. Lgr5 expression remained restricted to cell clusters within developing nephrons in the cortex until postnatal day 7, when expression was permanently silenced. PMID: 22999937
  43. While chief cells with Lgr5 transcriptional activity are present along the lesser curvature of the gastric oxyntic mucosa, they are not responsible for production of metaplasia. PMID: 22198711
  44. There is a stage-specific contribution of LGR5-expressing cells to luminal and basal epithelial lineages during postnatal mammary gland development in mice. PMID: 22926799
  45. Lgr5 has been identified as a marker of Wnt-regulated adult stem cell populations in the hair-follicle, intestine and stomach. A closely-related protein, Lgr6 marks adult stem cells responsible for fueling the renewal of the sebaceous gland and skin.[Review] PMID: 22002312
  46. This study showed here that Lgr5, a marker for adult stem cells, was expressed in a subset of supporting cells in the newborn and adult murine cochlea PMID: 22787049
  47. Lgr5 receptor-expressing intestinal stem cells function in the absence of Paneth cells PMID: 22355124
  48. Lgr5 is a marker for mitotically active crypt intestinal stem cells that exhibit exquisite sensitivity to canonical Wnt modulation, contribute robustly to homeostatic regeneration, and are quantitatively ablated by irradiation. PMID: 22190486
  49. Lgr5 expression is Myb-dependent. PMID: 21997934
  50. Lhx2 differentially regulates Sox9, Tcf4 and Lgr5 in hair follicle stem cells to promote epidermal regeneration after injury. PMID: 22028024

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Subcellular Location Cell membrane, Multi-pass membrane protein, Golgi apparatus, trans-Golgi network membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Expressed in the gonads, the adrenal gland, and in the brain. In the central nervous system expression is restricted to the olfactory bulb. In the adrenal gland detected only in the neural-crest derived chromaffin cells of the medulla, but not in the cell
Database Links

KEGG: mmu:14160

STRING: 10090.ENSMUSP00000020350

UniGene: Mm.42103

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