Mouse Lymphoid enhancer-binding factor 1(LEF1) ELISA kit

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Code CSB-EL012856MO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P27782
Lead Time 5-7 working days
Abbreviation LEF1
Protein Biological Process 1 Transcription/Transcription regulation
Target Name lymphoid enhancer-binding factor 1
Alias DKFZp586H0919, FLJ46390, TCF1ALPHA, T cell-specific transcription factor 1-alpha|TCF1-alpha
Species Mus musculus (Mouse)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 46.88 pg/mL-3000 pg/mL
Sensitivity 11.72 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. This protein can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LEF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 104
Range % 95-107
1:2 Average % 95
Range % 87-99
1:4 Average % 97
Range % 87-100
1:8 Average % 108
Range % 95-112
Recovery
The recovery of mouse LEF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 95-108
EDTA plasma (n=4) 100 93-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3000 2.208 2.183 2.196 2.066
1500 1.700 1.666 1.683 1.553
750 1.099 1.057 1.078 0.948
375 0.698 0.711 0.705 0.575
187.5 0.396 0.409 0.403 0.273
93.75 0.316 0.322 0.319 0.189
46.88 0.210 0.218 0.214 0.084
0 0.132 0.128 0.130  
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Function Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (By similarity). Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1.
Subcellular Location Nucleus
Protein Families TCF/LEF family
Tissue Specificity Lymphocytes. Found in distinct epithelial cell compartments of the skin and is abundant in the hair-producing progenitors of the follicle.
Database Links

KEGG: mmu:16842

STRING: 10090.ENSMUSP00000029611

UniGene: Mm.255219

Pathway Hippo signaling pathway
Melanogenesis
Wnt signaling pathway
Adherens junction

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