Mouse Lysosome-associated membrane glycoprotein 1(LAMP1) ELISA kit

Unavailable
Code CSB-EL012738MO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P11438
Lead Time 7-14 working days
Abbreviation LAMP1
Target Name lysosomal-associated membrane protein 1
Alias CD107a, LAMPA, LGP120,
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LAMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 105
Range % 99-109
1:2 Average % 86
Range % 81-89
1:4 Average % 92
Range % 87-98
1:8 Average % 89
Range % 85-93
Recovery
The recovery of mouse LAMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 91-102
EDTA plasma (n=4) 103 98-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
4000 2.712 2.847 2.780 2.638
2000 2.245 2.101 2.173 2.031
1000 1.467 1.428 1.448 1.306
500 0.843 0.835 0.839 0.697
250 0.451 0.472 0.462 0.320
125 0.277 0.283 0.280 0.138
62.5 0.218 0.222 0.220 0.078
0 0.143 0.141 0.142  
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Function Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
Subcellular Location Cell membrane, Single-pass type I membrane protein, Endosome membrane, Single-pass type I membrane protein, Lysosome membrane, Single-pass type I membrane protein, Late endosome
Protein Families LAMP family
Database Links

KEGG: mmu:16783

STRING: 10090.ENSMUSP00000033824

UniGene: Mm.16716

Pathway Autophagy
Secreted Extracellular Vesicles

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