Mouse Neudesin(NENF) ELISA kit

Instructions
Code CSB-EL015711MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name neuron derived neurotrophic factor
Alternative Names Nenf ELISA Kit; SpufNeudesin ELISA Kit; Neuron-derived neurotrophic factor ELISA Kit; Secreted protein of unknown function ELISA Kit; SPUF protein ELISA Kit
Abbreviation NENF
Uniprot No. Q9CQ45
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse NENF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 105
Range % 100-113
1:200 Average % 84
Range % 80-88
1:400 Average % 97
Range % 92-101
1:800 Average % 90
Range % 86-94
Recovery
The recovery of mouse NENF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 88 84-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
200 2.001 2.128 2.065 1.911
100 1.487 1.523 1.505 1.351
50 0.954 0.907 0.931 0.777
25 0.572 0.562 0.567 0.413
12.5 0.429 0.435 0.432 0.278
6.25 0.305 0.319 0.312 0.158
3.12 0.248 0.240 0.244 0.090
0 0.156 0.151 0.154  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Acts as a neurotrophic factor in postnatal mature neurons, enhancing neuronal survival
Gene References into Functions
  1. neudesin, which may be a negative regulator of sympathetic activity, could represent a novel regulator of the development of obesity and obesity-related metabolic dysfunctions. PMID: 25955136
  2. Neuron-derived neurotrophic factor (NENF) is an important central modulator of food intake. PMID: 23576617
  3. identification of neudesin, a novel mouse secreted protein showing neurotrophic activity in primary cultured mouse neurons PMID: 15605373
  4. The present findings have revealed new potential roles of neudesin in neural cell proliferation and neuronal differentiation. PMID: 16547973
  5. Neudesin is the first extracellular heme-binding protein that shows signal transducing activity by itself. The present findings may shed new light on the function of extracellular heme-binding proteins PMID: 18056703
  6. These findings suggest that neudesin plays a critical role in the early stage of adipocyte differentiation in which C/EBPbeta induces PPARgamma and C/EBPalpha expressions, by controlling the MAPK pathway. PMID: 19351598

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Subcellular Location Secreted, extracellular space
Protein Families Cytochrome b5 family, MAPR subfamily
Tissue Specificity In the embryo, expressed most abundantly in brain and spinal cord. Widely expressed in adult tissues including brain, heart, lung and kidney. In brain, expressed in neurons but not in glial cells (PubMed:15605373). In the hypothalamus is expressed primari
Database Links

KEGG: mmu:66208

STRING: 10090.ENSMUSP00000046311

UniGene: Mm.46444

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