Mouse Phospholipase A2, membrane associated(PLA2G2A) ELISA kit

Instructions
Code CSB-EL018091MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name phospholipase A2, group IIA (platelets, synovial fluid)
Alternative Names Pla2g2a ELISA Kit; Phospholipase A2 ELISA Kit; membrane associated ELISA Kit; EC 3.1.1.4 ELISA Kit; Enhancing factor ELISA Kit; EF ELISA Kit; GIIC sPLA2 ELISA Kit; Group IIA phospholipase A2 ELISA Kit; Phosphatidylcholine 2-acylhydrolase 2A ELISA Kit
Abbreviation PLA2G2A
Uniprot No. P31482
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.25 ng/mL-80 ng/mL
Sensitivity 0.312 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PLA2G2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 105
Range % 103-108
1:2 Average % 97
Range % 91-101
1:4 Average % 92
Range % 87-97
1:8 Average % 94
Range % 89-97
Recovery
The recovery of mouse PLA2G2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 103 96-106
EDTA plasma (n=4) 92 87-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
80 2.378 2.511 2.445 2.322
40 1.821 1.802 1.812 1.689
20 1.211 1.311 1.261 1.138
10 0.703 0.754 0.729 0.606
5 0.432 0.445 0.439 0.316
2.5 0.295 0.302 0.299 0.176
1.25 0.201 0.212 0.207 0.084
0 0.122 0.123 0.123  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides
Gene References into Functions
  1. PLA2 promotes pulmonary inflammation and systemic disease during Streptococcus pneumoniae infection. PMID: 28808157
  2. secretory PLA2s have important functions as genetic modifiers of inflammation and colon cancer. PMID: 27292189
  3. Dimethyl ester of bilirubin exhibits anti-inflammatory activity through inhibition of secretory phospholipase A2, lipoxygenase and cyclooxygenase. PMID: 27060751
  4. Polyozellin might play an important role in the modulation of sPLA2-IIA expression and activity in response to the inflammatory diseases. PMID: 26659873
  5. Progesterone-induced Acrosome Exocytosis Requires Sequential Involvement of Calcium-independent Phospholipase A2beta (iPLA2beta) and Group X Secreted Phospholipase A2 (sPLA2). PMID: 26655718
  6. Data show that the coordinated action of phospholipase A2 IIA (sPLA2-IIA) and 12-lipoxygenase (12-LO) promotes inflammatory arthritis. PMID: 26106157
  7. Pla2g2a sequence varies between tumor resistant and sensitive mouse strains. Some of these variations in the promoter region affect Pla2g2a expression and nonsense-mediated RNA decay also contributes to reducing Pla2g2a mRNA in tumor-sensitive strains. PMID: 24913681
  8. hydrolysis of the mitochondrial membrane by sPLA2-IIA yields inflammatory mediators (ie, lysophospholipids, fatty acids, and mtDNA) that promote leukocyte activation PMID: 25082876
  9. Ionomycin causes susceptibility to phospholipase A2 while temperature-induced increases in membrane fluidity fail: possible involvement of actin fragmentation. PMID: 24998360
  10. two of these isoforms, sPLA2 IIA and sPLA2 IIF, localize to the upper stratum granulosum and increase in response to experimental barrier perturbation. PMID: 24269828
  11. Investigation into the role of phosphatidylserine in modifying the susceptibility of lymphocytes to secretory phospholipase A(2) using cells deficient in the expression of scramblase. PMID: 22266334
  12. Treated mice exhibited upregulated sPLA2-IIa and cytokine gene transcription. PMID: 21519031
  13. results suggest that the tumor cell iPLAbeta-lysophosphatidic acid axis may represent a novel target for prostate cancer PMID: 20812222
  14. sPLA(2) may play a role in the development of the histologic changes produced by gastroduodenal reflux in esophageal mucosa. PMID: 20095996
  15. Secretory PLA2 plays a novel anti-inflammatory role in rheumatoid arthritis. PMID: 20432503
  16. calcium-independent phospholipase A2beta has a role in high glucose-induced activation of RhoA, Rho kinase, and CPI-17 in cultured vascular smooth muscle cells and vascular smooth muscle hypercontractility in diabetic animals PMID: 20086008
  17. Endogenous mouse sPLA(2) gene does not significantly affect HDL or atherosclerosis in mice. PMID: 12054745
  18. cPLA(2)alpha plays a discrete role in the collagen-stimulated production of TX and its inhibition has a therapeutic potential against thromboembolism, with potentially limited bleeding expected. PMID: 12163563
  19. Expression of enhancing factor/phospholipase A2 in skin results in abnormal epidermis and increased sensitivity to chemical carcinogenesis. PMID: 12673199
  20. the effect of group IIa and V PLA2s on H2O2-induced AA release is dependent upon the presence of cPLA2alpha and the activation of PKC and ERK1/2. Group IIa and V PLA2s are regulatory and cPLA2alpha is responsible for AA release. PMID: 12676927
  21. Hydrolysis of surfactant phosphatidylglycerol by group IIA secreted phospholipase A2 in vivo leads to an impairment of surfactant function in a mouse model of acute lung injury. PMID: 12847272
  22. Group V sPLA2 may play an important role in promoting atherosclerotic lesion development by modifying LDL particles in the arterial wall, thereby enhancing particle aggregation, retention, and macrophage uptake. PMID: 14962950
  23. experimental Plasmodium berghei infections of cytosolic PLA2 and PLA2IIA-deficient CL57B/6 mice resulted in cerebral malaria and rapid death; findings are expected to help clarify the involvement of various phospholipase A2s in malaria PMID: 15211007
  24. group VIA phospholipase A2 has a role in spermatozoa motility and male fertility PMID: 15252026
  25. macrophage sPLA2 IIa is a proatherogenic factor and regulates collagen production in the plaque PMID: 15576846
  26. the neuritogenic effect of sPLA2 is mediated by generation of LPC and subsequent activation of G2A PMID: 15927955
  27. detected low levels of enzyme activity in inbred strains containing sPLA2-IIA mutations; these strains were also associated with greater numbers of intestinal polyps PMID: 16007193
  28. the membranes of apoptotic cells become susceptible to sPLA(2) through a reduction in lipid-neighbor interactions that facilitates migration of phospholipids into the enzyme active site PMID: 17545239
  29. Current knowledge about the various links of sPLA2-IIA to cancer of the gastro-intestinal tract, and several models to illustrate its putative biological effects on tumor development.[REVIEW] PMID: 18508504
  30. Pla2g2a plays an important role, along with Muc2, in protection of the intestinal mucosa PMID: 19037975
  31. Interval and composite interval mapping along with permutation testing identified five significant susceptibility quantitative trait loci (QTLs) responsible for radiation induced tumour multiplicity in the small intestine. PMID: 19194513
  32. Results demonstrate that Pla2g2a attenuates colon tumorigenesis independent of Apc-germline mutations PMID: 19759415

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Subcellular Location Cell membrane, Peripheral membrane protein, Secreted
Protein Families Phospholipase A2 family
Tissue Specificity Mainly in the Paneth cells adjacent to the stem population in the small intestines. Also expressed in regenerating liver and hyperplastic esophageal epithelium.
Database Links

KEGG: mmu:18780

UniGene: Mm.4675

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