Mouse Protein APCDD1(APCDD1) ELISA kit

Instructions
Code CSB-EL001896MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name adenomatosis polyposis coli down-regulated 1
Alternative Names Apcdd1 ELISA Kit; Drapc1Protein APCDD1 ELISA Kit; Adenomatosis polyposis coli down-regulated 1 protein homolog ELISA Kit
Abbreviation APCDD1
Uniprot No. Q3U128
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse APCDD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-92
1:2Average %95
Range %91-98
1:4Average %95
Range %90-102
1:8Average %93
Range %84-98
Recovery
The recovery of mouse APCDD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)9792-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.712 2.699 2.706 2.523
20002.164 2.143 2.154 1.971
10001.403 1.428 1.416 1.233
5000.825 0.833 0.829 0.646
2500.599 0.611 0.605 0.422
1250.381 0.389 0.385 0.202
62.50.264 0.260 0.262 0.079
00.181 0.185 0.183
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Negative regulator of the Wnt signaling pathway. Inhibits Wnt signaling in a cell-autonomous manner and functions upstream of beta-catenin. May act via its interaction with Wnt and LRP proteins (By similarity).
Gene References into Functions
  1. these novel findings suggest that APCDD1 positively regulates adipogenic differentiation and that its down-regulation by miR-130 during diet-induced obesity may contribute to impaired adipogenic differentiation and obesity-related metabolic disease. PMID: 28242765
  2. Suggest that APCDD1 modulates the gene expression of Wnt- and EK-related signaling molecules at the cap stage of tooth development, and is involved in tooth cusp patterning by modulating epithelial rearrangement. PMID: 26170146
  3. mouse Drapc1 is a novel in vivo target gene of Wnt/beta-catenin signaling pathway PMID: 15465500
Subcellular Location Cell membrane, Single-pass type I membrane protein
Protein Families APCDD1 family
Database Links

KEGG: mmu:494504

STRING: 10090.ENSMUSP00000094302

UniGene: Mm.391102

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