Mouse SPARC-related modular calcium-binding protein 2(SMOC2) ELISA kit

Instructions
Code CSB-EL021843MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name SPARC related modular calcium binding 2
Alternative Names Smoc2 ELISA Kit; SPARC-related modular calcium-binding protein 2 ELISA Kit; Secreted modular calcium-binding protein 2 ELISA Kit; SMOC-2 ELISA Kit
Abbreviation SMOC2
Uniprot No. Q8CD91
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 93.8 pg/mL-6000 pg/mL
Sensitivity 23.45 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SMOC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 103
Range % 98-107
1:2 Average % 89
Range % 85-93
1:4 Average % 94
Range % 90-98
1:8 Average % 85
Range % 80-89
Recovery
The recovery of mouse SMOC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 91-102
EDTA plasma (n=4) 90 85-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
6000 2.641 2.546 2.594 2.480
3000 1.582 1.628 1.605 1.491
1500 0.914 0.957 0.936 0.822
750 0.609 0.593 0.601 0.487
375 0.375 0.384 0.380 0.266
187 0.268 0.279 0.274 0.160
93.8 0.167 0.161 0.164 0.050
0 0.116 0.112 0.114  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Can stimulate endothelial cell proliferation, migration, as well as angiogenesis (By similarity). Promotes matrix assembly and cell adhesiveness.
Gene References into Functions
  1. By using microRNA array, we identified a large number of microRNAs that showed different expression between wild-type Runx2 group and mutant groups. The aim of this study is to find out the effect of mmu-miR-1963, which was downregulated in all mutant Runx2 groups, on the ameloblast differentiation of LS8 cells PMID: 29233684
  2. SMOC1 and SMOC2 may mediate intercellular signaling and cell type-specific differentiation during gonad and reproductive tract development PMID: 19842175
  3. SMOC-2 is a novel angiogenic factor that potentiates angiogenic effects of growth factors PMID: 16774925
  4. SMOC-2 contributes to cell cycle progression by maintaining ILK activity during G1. PMID: 17989364
  5. SMOC-2, like other members of the BM40/SPARC family, acts as a regulator of cell-matrix interactions. PMID: 18582461
  6. results indicate that Smoc2 is a novel transcriptional target of activated Ahr. Perturbation of Smoc2 expression may mediate the adverse developmental effects of environmental aryl-hydrocarbon exposure PMID: 19146932

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Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane
Tissue Specificity Strongly expressed in ovary, followed by heart, muscle, spleen, brain, thymus, lung, liver, kidney, spleen, testis, ovary and skeletal muscle.
Database Links

KEGG: mmu:64074

STRING: 10090.ENSMUSP00000024660

UniGene: Mm.30162

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