Mouse Sclerostin(SOST) ELISA kit

Code CSB-EL022415MO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q99P68
Lead Time 5-7 working days
Abbreviation SOST
Protein Biological Process 1 Signaling Pathway
Target Name sclerosteosis
Alias VBCH, sclerostin
Species Mus musculus (Mouse)
Protein Biological Process 3 Wnt signaling pathway
Sample Types serum, plasma, tissue homogenates
Detection Range 93.75 pg/mL-6000 pg/mL
Sensitivity 23.4 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.
HGNC 13771
RGD 69358
MGI 1921749
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SOST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 82-91
1:2 Average % 101
Range % 98-105
1:4 Average % 87
Range % 84-91
1:8 Average % 100
Range % 95-105
Recovery
The recovery of mouse SOST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 85-94
EDTA plasma (n=4) 107 103-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
6000 2.904 2.869 2.887 2.720
3000 2.282 2.301 2.292 2.125
1500 1.507 1.536 1.522 1.355
750 0.921 0.902 0.912 0.745
375 0.607 0.612 0.610 0.443
187.5 0.356 0.362 0.359 0.192
93.75 0.248 0.268 0.258 0.091
0 0.165 0.169 0.167  
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Function Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Sclerostin family
Database Links

KEGG: mmu:74499

STRING: 10090.ENSMUSP00000001534

UniGene: Mm.265602

Pathway Wnt signaling pathway

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