Mouse Sclerostin(SOST) ELISA kit

Instructions
Code CSB-EL022415MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name sclerosteosis
Alternative Names VBCH, sclerostin
Abbreviation SOST
Uniprot No. Q99P68
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 93.75 pg/mL-6000 pg/mL
Sensitivity 23.4 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SOST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 82-91
1:2 Average % 101
Range % 98-105
1:4 Average % 87
Range % 84-91
1:8 Average % 100
Range % 95-105
Recovery
The recovery of mouse SOST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 85-94
EDTA plasma (n=4) 107 103-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
6000 2.904 2.869 2.887 2.720
3000 2.282 2.301 2.292 2.125
1500 1.507 1.536 1.522 1.355
750 0.921 0.902 0.912 0.745
375 0.607 0.612 0.610 0.443
187.5 0.356 0.362 0.359 0.192
93.75 0.248 0.268 0.258 0.091
0 0.165 0.169 0.167  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Sclerostin family
Database Links

KEGG: mmu:74499

STRING: 10090.ENSMUSP00000001534

UniGene: Mm.265602

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