Mouse Signal peptide, CUB and EGF-like domain-containing protein 1(SCUBE1) ELISA kit

Code CSB-EL020866MO
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
signal peptide, CUB domain, EGF-like 1
Alternative Names
Scube1 ELISA Kit; Signal peptide ELISA Kit; CUB and EGF-like domain-containing protein 1 ELISA Kit
Abbreviation
SCUBE1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SCUBE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 92  
Range % 88-95  
1:10 Average % 101  
Range % 96-104  
1:20 Average % 92  
Range % 87-95  
1:40 Average % 98  
Range % 92-101  
Recovery
The recovery of mouse SCUBE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-97  
EDTA plasma (n=4) 107 99-111  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.871 2.775 2.823 2.711  
5 2.054 1.989 2.022 1.910  
2.5 1.123 1.138 1.131 1.019  
1.25 0.645 0.675 0.660 0.548  
0.625 0.372 0.384 0.378 0.266  
0.312 0.287 0.277 0.282 0.170  
0.156 0.196 0.189 0.193 0.081  
0 0.113 0.111 0.112    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse SCUBE1 ELISA Kit was designed for the quantitative measurement of Mouse SCUBE1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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Target Background

Function
(From Uniprot)
Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.
Gene References into Functions
  1. SCUBE1 participates in platelet aggregation by bridging adjacent activated platelets in thrombosis. Blockade of soluble SCUBE1 might represent a novel antithrombotic strategy. PMID: 24833801
  2. SCUBE1 may be involved in the regulation of tubular cell proliferation and re-epithelialization during the resolution of kidney injury. PMID: 20042401
  3. These results support the dual roles of SCUBE1 on brain morphogenesis and cell-cell adhesions through its distinct domain function. PMID: 18303018
  4. During tooth development, Scube1 localized to the dental papilla of both incisor and molar teeth, suggestina a role for Scube1 during early craniofacial development. PMID: 19101956
Subcellular Location
Secreted. Cell membrane; Peripheral membrane protein.
Database Links

KEGG: mmu:64706

UniGene: Mm.40393

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