Mouse Sodium/potassium-transporting ATPase subunit alpha-2(ATP1A2) ELISA kit

Instructions
Code CSB-EL002323MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
Alternative Names Atp1a2 ELISA Kit; Sodium/potassium-transporting ATPase subunit alpha-2 ELISA Kit; Na(+)/K(+) ATPase alpha-2 subunit ELISA Kit; EC 7.2.2.13 ELISA Kit; Na(+)/K(+) ATPase alpha(+) subunit ELISA Kit; Sodium pump subunit alpha-2 ELISA Kit
Abbreviation ATP1A2
Uniprot No. Q6PIE5
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ATP1A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %96
Range %90-100
1:2Average %104
Range %100-108
1:4Average %84
Range %80-88
1:8Average %90
Range %86-94
Recovery
The recovery of mouse ATP1A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8984-93
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1OD2AverageCorrected
20002.613 2.537 2.575 2.439
10002.148 2.156 2.152 2.016
5001.478 1.427 1.453 1.317
2500.841 0.875 0.858 0.722
1250.524 0.569 0.547 0.411
62.50.307 0.329 0.318 0.182
31.250.226 0.239 0.233 0.097
00.131 0.140 0.136
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity).
Gene References into Functions
  1. proof of concept study demonstrates that reduced expression of the alpha2 isoform in the spinal cord is protective following spinal cord injury PMID: 28886701
  2. Atp1a2 does contribute to alcohol-related behaviors, but the effect is modest with a clear dependence on sex. PMID: 27814792
  3. Atp1alpha2 is preferentially expressed with Atp1beta2 in T-tubules of cardiac myocytes, forming alpha2beta2 heterodimers. PMID: 27624940
  4. study provides evidence that muscle beta-catenin is crucial for maintenance of resting membrane potential(RMP)of skeletal muscle cells; muscle cells lacking beta-catenin show decreased expression of alpha2 subunit of Na,K-ATPase, a probable mechanism by which beta-catenin regulates RMP and affects normal function of skeletal muscle PMID: 24674304
  5. Heart expression of alpha2 does not play a role in the regulation of basal cardiovascular function or blood pressure; however, heart expression of alpha2 plays a role in the hypertrophic response to pressure overload. PMID: 23436327
  6. Atp1a2 has a more prominent role (vs. Atp1a1) in modulating cardiac myocyte sarcoplasmic reticulum Ca(2+) release. PMID: 22739122
  7. The ouabain-binding site of either the alpha1 or alpha2 Na,K-ATPase subunit does not play an essential role in the development of DOCA-salt hypertension in a mouse model PMID: 22258333
  8. Central sodium chloride causes hypertension in mice. The blood pressure response is mediated by the ouabain-like substance in the brain, specifically by its binding to the alpha(2)-isoform of the Na,K-ATPase. PMID: 21856907
  9. Atp1a2 expression in heart and vascular smooth muscle is not essential for regulation of basal systolic blood pressure. PMID: 21856916
  10. Ion channel NKCC1 and alpha2 Na, K-ATPase played important roles in the inner ear potassium recycling. PMID: 17111813
  11. Both protein and mRNA expression of alpha1 and alpha2 isoforms of Na,K-ATPase and NKCC1 in the lateral wall were dramatically reduced following a long-term deafening PMID: 21094218
  12. The cardiotonic steroid-binding site of the alpha2 Na,K-ATPase plays a role in maintaining normal SBP during pregnancy PMID: 20940714
  13. The Na,K-ATPase alpha(2) subunit is expressed during development when the t-tubules form. These results suggest that the alpha(2) isoform may serve, in part, a physiological role in the muscle t-tubules. PMID: 12397396
  14. Na,K-ATPase alpha2 isoform could be important in the modulation of neuronal activity in the neonate PMID: 12458206
  15. Ca2+ signaling is regulated by the expression of this protein as studied in knockout mice PMID: 12763872
  16. Heterozygote Atp1a2 mutant adult mice showed increased fear/anxiety behavior and enhanced neuronal activity in the amygdala and piriform cortex after conditioned fear stimuli; homozygotes died just post-birth and showed neuronal apoptosis in these regions PMID: 12805306
  17. the alpha2 Na,K-ATPase isozyme mediates ouabain-induced cardiac contractility in mice PMID: 14559919
  18. individual alpha-isoforms of the Na(+)/K(+)-ATPase differ functionally and that the alpha(2)-isoform couples more strongly to activation-relaxation pathways in vascular smooth muscle PMID: 14627611
  19. the alpha2-isoform of Na-K-ATPase mediates the ouabain-induced increase in vascular contractility and could play a role in the development and maintenance of ouabain-induced hypertension. PMID: 15458945
  20. We propose a model for functional coupling between the Na+, K+-ATPase alpha2 subunit and KCC2, which excludes Cl- from the cytosol in respiratory center neurons. PMID: 15564586
  21. Accordingly, in salt-dependent hypertension, EOLC probably increases vascular resistance and blood pressure by reducing alpha2 Na(+) pump activity and promoting Ca(2)(+) entry via NCX in myocytes. PMID: 16166162
  22. Leu-27 and Ala-35 are essential for targeting/tethering Na+ pump alpha2 subunits to plasma membrane (PM) microdomains overlying "junctional" sarco-/endoplasmic reticulum PMID: 16524882
  23. Learning and memory deficits observed in Na,K-ATPase alpha2 and alpha3 mice reveal Na,K-ATPase to be an important factor in the functioning of pathways associated with spatial learning. PMID: 17234593
  24. Present 3D structural model of Atp1a2. PMID: 17552883
  25. double heterozygote deletion of NKCC1 with alpha(2)-Na,K-ATPase demonstrates a striking preservation of hearing threshold both initially and with age. PMID: 17674100
  26. Coupling between the two respiration-related rhythm generators did not function in the normal manner in Atp1a2(-/)(-) mice. PMID: 17690149
  27. FGF23 transgenic mice display differentially expressed transcript levels of several genes (Npt2a, Pdzk1, Atp1a2) essential in renal Pi regulation. PMID: 17911089
  28. differential distribution of alpha-subunits of the Na+/K+-ATPase in subicular interneurones and pyramidal cells PMID: 17947306
  29. studies of the behavioral aspects of alpha1, alpha2, and alpha 3 deficient mice show that haploinsufficiency of both alpha2 and alpha 3 isoforms result in behavioral defects. PMID: 18044013
  30. Our findings support the concept of a subsarcolemmal space where the alpha 2-isoform coupled with Na+-Ca2+ exchanger modulates Ca2+-store function and Ca2+ entry PMID: 18192219
  31. ACTH-induced hypertension is dependent on the ouabain-binding site of the alpha2-Na+-K+-ATPase subunit. PMID: 18487447
  32. Chronic downregulation of Na(+)-K(+)-ATPase alpha(2)-isoform expression by heterozygous knockout increases the pressor response to increased CSF [Na] and activates the brain renin-angiotensin system. PMID: 19244589

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Subcellular Location Membrane, Multi-pass membrane protein, Cell membrane, Multi-pass membrane protein
Protein Families Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIC subfamily
Database Links

KEGG: mmu:98660

STRING: 10090.ENSMUSP00000083077

UniGene: Mm.207432

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