Mouse Soluble Toll-like Receptor 2,sTLR2 ELISA Kit

Instructions
Code CSB-E14274m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name toll-like receptor 2
Alternative Names CD282, TIL4, toll/interleukin 1 receptor-like 4
Abbreviation TLR2
Uniprot No. Q9QUN7
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse sTLR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 85-93
1:2 Average % 95
Range % 90-100
1:4 Average % 103
Range % 99-107
1:8 Average % 90
Range % 86-94
Recovery
The recovery of mouse sTLR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 85 80-90
EDTA plasma (n=4) 93 89-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
40 2.718 2.566 2.642 2.452
20 2.004 1.905 1.955 1.765
10 1.320 1.357 1.339 1.149
5 0.847 0.863 0.855 0.665
2.5 0.500 0.481 0.491 0.301
1.25 0.351 0.339 0.345 0.155
0.625 0.269 0.278 0.274 0.084
0 0.186 0.194 0.190  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity)
Subcellular Location Cell membrane, Single-pass type I membrane protein, Cytoplasmic vesicle, phagosome membrane, Single-pass type I membrane protein, Membrane raft
Protein Families Toll-like receptor family
Tissue Specificity Detected in a macrophage cell line, smooth muscle, lung, spleen, thymus, brain and adipose tissue. Cell surface expression detected in lung alveolar macrophages, dendritic macrophages and at lower levels in lung macrophages (at protein level) (PubMed:1936
Database Links

KEGG: mmu:24088

STRING: 10090.ENSMUSP00000029623

UniGene: Mm.87596

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