Code | CSB-E04723m |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SDF-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 96 | ||||
Range % | 92-100 | |||||
1:2 | Average % | 102 | ||||
Range % | 99-105 | |||||
1:4 | Average % | 89 | ||||
Range % | 87-91 | |||||
1:8 | Average % | 94 | ||||
Range % | 90-98 |
The recovery of mouse SDF-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 103 | 99-107 | ||||
EDTA plasma (n=4) | 95 | 90-100 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The product CSB-E04723m is a sandwich ELISA kit developed to measure concentrations of mouse CXCL12 in serum, plasma, cell culture supernates, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of CXCL12 in samples. The color intensity is measured at 450 nm via a microplate reader.
CXCL12, also called SDF-1, is a homeostatic CXC chemokine that regulates hematopoietic cell trafficking and secondary lymphoid tissue architecture. CXCL12 exerts its various effects through its receptors, CXCR4 and CXCR7. The CXCL12/CXCR4/CXCR7 axis plays a pivotal role in tumor development, survival, angiogenesis, metastasis, and tumor microenvironment. CXCL12 is known to contribute a lot to renal development, especially in renal vascular formation. It is involved in various kidney diseases, such as acute kidney injury (AKI), diabetic kidney disease (DKD), lupus nephritis, kidney transplantation, and renal cell carcinoma (RCC).
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