Mouse Syndecan-3(SDC3) ELISA kit

Instructions
Code CSB-EL020890MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name syndecan 3
Alternative Names Sdc3 ELISA Kit; Kiaa0468 ELISA Kit; Syndecan-3 ELISA Kit; SYND3 ELISA Kit
Abbreviation SDC3
Uniprot No. Q64519
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SDC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %88-96
1:2Average %89
Range %85-97
1:4Average %103
Range %99-107
1:8Average %89
Range %84-94
Recovery
The recovery of mouse SDC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8784-89
EDTA plasma (n=4)9188-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.958 2.779 2.869 2.704
20002.045 2.225 2.135 1.970
10001.462 1.424 1.443 1.278
5000.803 0.817 0.810 0.645
2500.578 0.547 0.563 0.398
1250.322 0.346 0.334 0.169
62.50.266 0.271 0.269 0.104
00.159 0.171 0.165
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Cell surface proteoglycan that may bear heparan sulfate. May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism (By similarity).
Gene References into Functions
  1. Syndecan-3 ablation unexpectedly improves regeneration in repeatedly injured muscle and in dystrophic mice, accompanied by the persistence of sublaminar and interstitial, proliferating myoblasts PMID: 27757223
  2. This study shows a novel role for syndecan-3 in inflammation PMID: 25015005
  3. Syndecan-3 limits cocaine intake by modulating the effects of glial-cell-line-derived neurotrophic factor, which uses syndecan-3 as an alternative receptor. PMID: 23736082
  4. This study showed that the melanocortin agonist Melanotan II (MTII) potently suppresses food intake and activates the hypothalamic paraventricular nuclei (PVN) in SDC-3(-/-) mice based on c-fos immunoreactivity PMID: 20923696
  5. Syndecan-3 may directly transduce Glial cell line-derived neurotrophic factor (GDNF) family ligands (GFL) signals or serve as a coreceptor, presenting GFLs to the signaling receptor RET. PMID: 21200028
  6. The Syndecan-3 interacts with Notch and is required for Notch processing by ADAM17/tumor necrosis factor-alpha-converting enzyme (TACE) and signal transduction. PMID: 20696709
  7. Identification of the core protein carrying the Tn antigen in mouse brain: specific expression on syndecan-3. PMID: 11831359
  8. Syndecan-3 mRNA transcripts are expressed by reactive astrocytes in the region surrounding the necrotic brain tissue, exhibiting peak levels at day 7 after cryo-injury. PMID: 12112370
  9. syndecan-3 acts as an important modulator of synaptic plasticity that influences hippocampus-dependent memory. PMID: 12359158
  10. Data support the hypothesis that syndecan-3 modulates melanocortin activity via facilitation of agouti-related protein. PMID: 12851299
  11. hydrolysis is gamma-secretase dependent and modulates cytosolic signaling PMID: 14504279
  12. Syndecan-3(-/-) mice exhibit a novel form of muscular dystrophy characterized by impaired locomotion, fibrosis, and hyperplasia of myonuclei and satellite cells, suggesting a role of syndecan-3 in muscle development PMID: 15371336
  13. syndecan-3 has a role in the regulation of body weight and in obesity PMID: 15520868
  14. Data show that massive cell death of cultured dorsal root ganglion (DRG) neurons from mice deficient in the N-syndecan gene as compared with wild-type controls. PMID: 18766019

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Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Syndecan proteoglycan family
Database Links

KEGG: mmu:20970

STRING: 10090.ENSMUSP00000065877

UniGene: Mm.206536

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