Mouse TGF-beta receptor type-2(TGFBR2) ELISA kit

Instructions
Code CSB-EL023452MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name transforming growth factor, beta receptor II (70/80kDa)
Alternative Names Tgfbr2 ELISA Kit; TGF-beta receptor type-2 ELISA Kit; TGFR-2 ELISA Kit; EC 2.7.11.30 ELISA Kit; TGF-beta type II receptor ELISA Kit; Transforming growth factor-beta receptor type II ELISA Kit; TGF-beta receptor type II ELISA Kit; TbetaR-II ELISA Kit
Abbreviation TGFBR2
Uniprot No. Q62312
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways (By similarity).
Gene References into Functions
  1. the molecular actions of TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis PMID: 29352019
  2. altered Tgfbeta signaling in cultured mouse and human enteroids supports further the in vivo data and reveals a critical role for Tgfbeta signaling in generating precursor secretory cells. Overall, our data reveal a key role for Tgfbeta signaling in regulating ISCs clonal dynamics and differentiation, with implications for cancer, tissue regeneration, and inflammation. PMID: 27791005
  3. In young Fbn1(C1039G/+) mice, aortopathy develops in the absence of detectable alterations in smooth muscle cell (SMC) TGF-beta signaling. Loss of physiologic SMC TGF-beta signaling exacerbates MFS-associated aortopathy. Our data support a protective role for SMC TGF-beta signaling during early development of MFS-associated aortopathy. PMID: 28119285
  4. The present study demonstrates that TGFbeta responsiveness in the myeloid compartment is critical in controlling autoimmune encephalomyelitis, and that its specific deletion in monocyte-derived dendritic cells causes severe experimental autoimmune encephalomyelitis without remission. PMID: 27479807
  5. These results suggest proliferation and maturation of immature osteoblasts requires Tgfbr2 signaling and that decreased bone volume in Osx-Cre;Tgfbr2(fl/fl) mice is likely due to fewer mature osteoblasts. PMID: 28043895
  6. The authors identify ELMO1 as a novel target of TGFbeta signaling and show that restoration of Tgfbr2 results in a complete block of ELMO1 in vivo. Knocking down Elmo1 impairs metastasis of carcinoma cells to the lung, thereby providing insights into the mechanisms of progression of Tgfbr2-deficient invasive transition zone squamous cell carcinoma. PMID: 28219480
  7. Cell-intrinsic TGFbeta-RII signaling limits CD4 T cell proliferation but not prototypical T helper subset differentiation early after chronic lymphocytic choriomeningitis virus infection. PMID: 27599295
  8. Knockout of the TGFbeta receptor in proopiomelanocortin neurons counteracts the hypertensive effect of not only TGFbeta but also mtDNA excess, while the hypertensive action of central mtDNA can be blocked pharmacologically by a TGFbeta receptor antagonist or genetically by TGFbeta receptor knockout. PMID: 27894066
  9. Postnatal disruption of Tgfbr2 gene compromises extracellular matrix properties and promotes aortic dissection, which can be prevented by rapamycin treatment. PMID: 26988590
  10. Gasdermin C is upregulated by inactivation of Tgfbr2 in the presence of mutated Apc, promoting colorectal cancer cell proliferation. PMID: 27835699
  11. Epithelial TGFbeta signaling via TGFBR2 does not contribute to the development of liver fibrosis or formation of hepatocellular carcinomas in mice, but restricts cholangiocyte proliferation to prevent cholangiocarcinoma development. PMID: 26627606
  12. The removal of Tgfbr2 and treatment with losartan both delayed the progression of articular cartilage degeneration induced by medial meniscus (DMM) compared with control littermates. PMID: 26355014
  13. Data (including data from studies in transgenic mice) suggest intraislet pancreatic duct cells are capable of giving rise to insulin-secreting beta-cells; Tgfbr2/transforming growth factor-beta type II receptor appears to be involved in this process. PMID: 26505114
  14. Elimination of TGF-betaIIR is not sufficient to completely prevent liver fibrosis. TGF-beta-independent mechanism of type I collagen production and suggest connective tissue growth factor as its potent mediator. PMID: 25108224
  15. Overexpression of TGFbeta1 promotes pulmonary inflammation, apoptosis and mortality via TGFbetaR2 in the developing mouse lung. PMID: 25591994
  16. Loss of smooth muscle cells Tgfbr2 disrupts TGF-beta signaling, acutely alters SMC gene expression, and rapidly results in severe and durable aortopathy. PMID: 26494233
  17. Increased Tgfbr2 expression is associated with pulmonary fibrosis. PMID: 26216407
  18. Suggest that miR-370 acting via TbetaRII might play a potential role in hepatic IR injury, and inhibition of miR-370 efficiently attenuated the damage to the liver. PMID: 24351048
  19. skeletal phenotype of mice carrying a mutation in the TGF-beta type 2 receptor associated with severe Loeys-Dietz syndrome in humans: Cortical bone showed significantly reduced tissue area, bone area, and cortical thickness with increased eccentricity PMID: 26173585
  20. Expression of microRNA-211 is regulated by the key melanocyte transcription factor MITF and regulates pigmentation by targeting the TGF-beta receptor 2. PMID: 25444235
  21. TGFBR2-dependent molecular crosstalk between neural and endothelial cells during brain vessel development was discovered. PMID: 24990151
  22. TGF-beta signaling plays an important role in dentin formation and pulp protection. Furthermore, our findings may provide new insight into possible mechanisms underlying human hereditary dentin disorders and reparative dentin formation. PMID: 25818583
  23. taken together, indicate that a simple genetic alteration in the TGFbeta signaling pathway in the inflamed and regenerating intestinal mucosa can cause invasive intestinal tumors. PMID: 25687406
  24. phosphorylation of tyrosines within the TbetaRII tail was essential for SMAD-dependent fibrotic signaling within cells of the kidney collecting duct PMID: 24983314
  25. Conditional deletion of Tgfbr2 in keratin 14-positive stratified epithelia causes ocular surface epithelial hyperplasia and conjunctival goblet cell expansion that invaginates into the subconjunctival stroma in the mouse eye. PMID: 25377551
  26. The TGFbetaRII-mediated signaling in thymic epithelial cells inhibits their development into Hassall's corpuscles in mice PMID: 23929912
  27. CD4Foxp3 thymocytes from mice with the Tgfbr2 deletion expressed high levels of CXCR3. PMID: 24165986
  28. basal TGF-beta signaling in smooth muscle promotes postnatal aortic wall homeostasis and impedes disease progression PMID: 24401272
  29. TGFbeta regulates epithelial-mesenchymal interactions through WNT signaling activity to control muscle development in the soft palate. PMID: 24496627
  30. Inhibition of TGFbeta signaling (deletion of the Tgfbr2 gene in chondrocytes) resulted in up-regulation of Runx2, Mmp13, and Adamts5 expression in articular cartilage tissue and progressive osteoarthritis development in TGFbetaRII(Col2ER) mice. PMID: 23982761
  31. Data indicate thate TGFbeta activates the ABL1 pathway in the absnce of TGFbeta receptor type II (TbetaRII) during tongue development. PMID: 23950180
  32. Osx-Cre;Tgfbr2(fl/fl) mice were generated (Tgfbr2(cko)) and post-natal tooth development was compared in Tgfbr2(cko) and control littermates. PMID: 23933490
  33. Spatial and temporal expression of Tgfbr2 is regulated during mouse forelimb development. PMID: 23231014
  34. TbetaRII-deficient mice showed a lower level of developmental apoptosis of retinal neurons. PMID: 23986258
  35. Reduced cholesterol synthesis is observed in Tgfbr2 mutant palatal tissue. PMID: 23060211
  36. This study uncovers a previously unrecognized dominant function of the dnTGFbetaRII in CD8(+) T cell proliferation and cellular transformation PMID: 23686479
  37. Increased copy number of dnTGFbetaRII is critical for development of lymphoma-like T cell infiltration. PMID: 23145171
  38. gross alterations in dorsal vertebrae were apparent by E16.5days in Tgfbr2 mutants PMID: 22369999
  39. Loss of Tgfbr2 in hypertrophic chondrocytes using Col10a1-Cre mice resulted in delays in both the hypertrophic conversion of proliferating chondrocytes and terminal chondrocyte differentiation PMID: 22885149
  40. IRF6 and SMAD4 synergistically regulate the fate of the medial edge epithelium(MEE), and TGFbeta-mediated Irf6 activity is responsible for MEE degeneration during palatal fusion in mice. PMID: 23406900
  41. c-Cbl conjugates neural precursor cell-expressed, developmentally downregulated 8 (NEDD8), a ubiquitin-like protein, to TbetaRII at Lys556 and Lys567. PMID: 23290524
  42. Deficiency of the TGF-beta type II receptor protected proximal tubule epithelial cells from apoptosis. PMID: 23160515
  43. TGFbetaR2 signaling in dendritic cells is critical to control autoimmunity through both regulatory T (Treg) cell-dependent and -independent mechanisms. PMID: 22972928
  44. TGFbeta signalling is essential in vascular endothelial cells for maintaining vascular integrity at the angiogenic front as it migrates into developing neural tissues in early postnatal life. PMID: 22745736
  45. Results establish a novel role for TGFbeta signaling in expression of genes for alternatively activated macrophages. Lack of TbetaRII signaling may reduce the anti-inflammatory M2 phenotype of macrophages because of reduced expression of these products. PMID: 22703233
  46. Treatment of PyMT/Tgfbr2(KO) mice with anti-IL-17 Ab decreased carcinoma growth and metastatic burden. PMID: 22408746
  47. We conclude that Tgfbr2 in myeloid cells has a negative role in the regulation of anti-tumorigenic functions of these cells PMID: 22685318
  48. The TbetaRII/monocyte-chemoattractant protein (MCP)-5 axis is an essential crossroad for joint development and endochondral growth. PMID: 22814601
  49. TbetaRII may determine the distinct roles of TGF-beta1 in inflammation and fibrogenesis in a mouse model of ureteral obstruction induced nephropathy. PMID: 22190171
  50. Data show that dual Dicer-deficient and TGFbetaRII-deficient mice show defects in both TGFbeta-mediated tolerance and miR production. PMID: 22225602

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Subcellular Location Cell membrane, Single-pass type I membrane protein, Membrane raft
Protein Families Protein kinase superfamily, TKL Ser/Thr protein kinase family, TGFB receptor subfamily
Tissue Specificity Widely expressed in adult. Expressed primarily in mesenchyme and epidermis of the midgestational fetus.
Database Links

KEGG: mmu:21813

STRING: 10090.ENSMUSP00000062333

UniGene: Mm.172346

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