Mouse Tenomodulin(TNMD) ELISA kit

Instructions
Code CSB-EL024007MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name tenomodulin
Alternative Names Tnmd ELISA Kit; Chm1l ELISA Kit; Tenomodulin ELISA Kit; TeM ELISA Kit; mTeM ELISA Kit; Chondromodulin-1-like protein ELISA Kit; ChM1L ELISA Kit; mChM1L ELISA Kit; Chondromodulin-I-like protein ELISA Kit; Myodulin ELISA Kit; Tendin ELISA Kit
Abbreviation TNMD
Uniprot No. Q9EP64
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 9.38 pg/mL-600 pg/mL
Sensitivity 2.34 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNMD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 108
Range % 85-112
1:2 Average % 103
Range % 94-106
1:4 Average % 97
Range % 87-102
1:8 Average % 102
Range % 96-105
Recovery
The recovery of mouse TNMD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 92-105
EDTA plasma (n=4) 99 88-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
600 2.238 2.176 2.207 2.036
300 1.574 1.622 1.598 1.427
150 1.018 1.061 1.040 0.869
75 0.673 0.723 0.698 0.527
37.5 0.501 0.524 0.513 0.342
18.75 0.412 0.420 0.416 0.245
9.38 0.286 0.278 0.282 0.111
0 0.173 0.168 0.171  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function May be an angiogenesis inhibitor.
Gene References into Functions
  1. Tnmd is required for prevention of adipocyte accumulation and fibrovascular scar formation during early tendon healing. PMID: 29022912
  2. The results of enhanced tenogenic differentiation and neotendon formation indicated that Tnmd may serve not only as a tenogenic marker but also as a positive regulator of MSCs tenogenic differentiation, which might be applied to MSCs-mediated tendon regeneration. PMID: 27098985
  3. our study demonstrates that Tnmd is required for proper tendon tissue adaptation to endurance running and aids in better understanding of the structural-functional relationships of tendon tissues. PMID: 28566251
  4. In vitro comparison of tenogenic differentiation potentials among bone marrow derived stem cells (BMSCs), adipose derived stem cells (ASCs), dermal fibroblasts (DFs) and tenocytes (TCs) of the same Tnmd transgenic mice. BMSCs exhibited the best tenogenic potential than other three cell types. PMID: 27777108
  5. Upon high-fat diet feeding, transgenic mice overexpressing Tnmd develop increased epididymal white adipose tissue (eWAT) mass, and preadipocytes derived from Tnmd transgenic mice display greater proliferation, consistent with elevated adipogenesis. PMID: 26880110
  6. Loss of Tnmd affects significantly the self-renewal and senescence properties, but not the multipotential of tendon stem cells. PMID: 25351164
  7. In our study of MM development, the laminar structure regulating TeM also prevents vascular invasion during the formation of compartment of the MM. PMID: 25107480
  8. Results suggest that tenomodulin (Tnmd) is a regulator of tenocyte proliferation and is involved in collagen fibril maturation but do not confirm an in vivo involvement of Tnmd in angiogenesis. PMID: 15632070
  9. The expression domains of ChM-I and TeM during vertebrate development incorporate the typical avascular regions of the mesenchymal tissues. PMID: 18239943
  10. Tenomodulin is expressed abundantly in the elastin-rich subendothelial outer layer of the normal chordae tendineae cordis. PMID: 18838562

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Subcellular Location Membrane, Single-pass type II membrane protein, Nucleus envelope
Protein Families Chondromodulin-1 family
Tissue Specificity Widely expressed with highest expression in tendons and ligaments, in the diaphragm, eye and skeletal muscle. Expressed in neuronal cells of all brain regions. Very low expression, if any, in glial cells.
Database Links

KEGG: mmu:64103

STRING: 10090.ENSMUSP00000033602

UniGene: Mm.46221

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