Product Details

Target Name

tenomodulin

Alternative Names

Tnmd ELISA Kit; Chm1l ELISA Kit; Tenomodulin ELISA Kit; TeM ELISA Kit; mTeM ELISA Kit; Chondromodulin-1-like protein ELISA Kit; ChM1L ELISA Kit; mChM1L ELISA Kit; Chondromodulin-I-like protein ELISA Kit; Myodulin ELISA Kit; Tendin ELISA Kit

Abbreviation

TNMD

Uniprot No.

Q9EP64

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

9.38 pg/mL-600 pg/mL

Sensitivity

2.34 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNMD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	108
	Range %	85-112
1:2	Average %	103
	Range %	94-106
1:4	Average %	97
	Range %	87-102
1:8	Average %	102
	Range %	96-105

Recovery

The recovery of mouse TNMD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	92-105
EDTA plasma (n=4)	99	88-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
600	2.238	2.176	2.207	2.036
300	1.574	1.622	1.598	1.427
150	1.018	1.061	1.040	0.869
75	0.673	0.723	0.698	0.527
37.5	0.501	0.524	0.513	0.342
18.75	0.412	0.420	0.416	0.245
9.38	0.286	0.278	0.282	0.111
0	0.173	0.168	0.171

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

7-14 working days

Target Data

Function	May be an angiogenesis inhibitor.
Gene References into Functions	Tnmd is required for prevention of adipocyte accumulation and fibrovascular scar formation during early tendon healing. PMID: 29022912 The results of enhanced tenogenic differentiation and neotendon formation indicated that Tnmd may serve not only as a tenogenic marker but also as a positive regulator of MSCs tenogenic differentiation, which might be applied to MSCs-mediated tendon regeneration. PMID: 27098985 our study demonstrates that Tnmd is required for proper tendon tissue adaptation to endurance running and aids in better understanding of the structural-functional relationships of tendon tissues. PMID: 28566251 In vitro comparison of tenogenic differentiation potentials among bone marrow derived stem cells (BMSCs), adipose derived stem cells (ASCs), dermal fibroblasts (DFs) and tenocytes (TCs) of the same Tnmd transgenic mice. BMSCs exhibited the best tenogenic potential than other three cell types. PMID: 27777108 Upon high-fat diet feeding, transgenic mice overexpressing Tnmd develop increased epididymal white adipose tissue (eWAT) mass, and preadipocytes derived from Tnmd transgenic mice display greater proliferation, consistent with elevated adipogenesis. PMID: 26880110 Loss of Tnmd affects significantly the self-renewal and senescence properties, but not the multipotential of tendon stem cells. PMID: 25351164 In our study of MM development, the laminar structure regulating TeM also prevents vascular invasion during the formation of compartment of the MM. PMID: 25107480 Results suggest that tenomodulin (Tnmd) is a regulator of tenocyte proliferation and is involved in collagen fibril maturation but do not confirm an in vivo involvement of Tnmd in angiogenesis. PMID: 15632070 The expression domains of ChM-I and TeM during vertebrate development incorporate the typical avascular regions of the mesenchymal tissues. PMID: 18239943 Tenomodulin is expressed abundantly in the elastin-rich subendothelial outer layer of the normal chordae tendineae cordis. PMID: 18838562 Show More Hide All
Subcellular Location	Membrane, Single-pass type II membrane protein, Nucleus envelope
Protein Families	Chondromodulin-1 family
Tissue Specificity	Widely expressed with highest expression in tendons and ligaments, in the diaphragm, eye and skeletal muscle. Expressed in neuronal cells of all brain regions. Very low expression, if any, in glial cells.
Database Links	KEGG: mmu:64103 STRING: 10090.ENSMUSP00000033602 UniGene: Mm.46221

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Check Code：

Code	CSB-EL024007MO
Size	96T,5×96T,10×96T
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Mouse Tenomodulin(TNMD) ELISA kit

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