Mouse Toll-like receptor 5,TLR-5 ELISA kit

Instructions
Code CSB-E12744m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name toll-like receptor 5
Alternative Names Tlr5 ELISA Kit; Toll-like receptor 5 ELISA Kit
Abbreviation TLR5
Uniprot No. Q9JLF7
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TLR-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %82
Range %85-87
1:2Average %102
Range %97-106
1:4Average %99
Range %96-103
1:8Average %92
Range %87-96
Recovery
The recovery of mouse TLR-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8883-94
EDTA plasma (n=4)9590-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
50002.745 2.955 2.850 2.715
25002.021 2.118 2.070 1.935
12501.333 1.353 1.343 1.208
6250.776 0.835 0.806 0.671
3120.564 0.526 0.545 0.410
1560.324 0.312 0.318 0.183
780.229 0.241 0.235 0.100
00.131 0.139 0.135
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).
Gene References into Functions
  1. hematopoietic TLR5 deficiency inhibits atherosclerotic lesion formation by attenuated macrophage accumulation and defective T-cell responsiveness. PMID: 28202909
  2. Denatured flagellin suppressed the production of the pro-inflammatory cytokines induced by intact flagellin or Pseudomonas aeruginosa both in vitro and in vivo, probably by blocking TLR5. PMID: 29589550
  3. Data show that Toll-like receptor 5 (TLR5) plays a role in radiation-induced apoptosis, and CBLB502 pre-treatment could reduce the apoptosis. PMID: 28407032
  4. These results suggest that HMGB1-modulated TLR5 signaling is responsible for pain hypersensitivity. PMID: 27760316
  5. data directly demonstrate that nasal epithelial GM-CSF contributes to TLR5-mediated modulation of airway DCs and a subsequent IgA response. PMID: 28522600
  6. Distinctive Recognition of Flagellin by Human and Mouse Toll-Like Receptor 5 PMID: 27391968
  7. The activation of NLRC4 by flagellin downregulated the flagellin-induced and TLR5-mediated immune responses against flagellin. PMID: 25914934
  8. TLR5 but not NLRC4 is required for S. pneumoniae FliC-induced protection. PMID: 27546231
  9. TLR5 mediates CD172a(+) intestinal lamina propria dendritic cell induction of Th17 cells. PMID: 26907705
  10. TLR5 activation plays an important role in the induction of podocyte apoptosis PMID: 26914743
  11. TLR5 gene knockout impairs some effects of weight-reduction in diet-induced obesity (DIO). The glucose intolerance in DIO TLR5(-/-) mice was more significant than that in DIO C57BL/6 mice. PMID: 26681840
  12. The results suggest that caveolin-1/TLR5 signaling plays a key role in age-associated innate immune responses and that FlaB-PspA stimulation of TLR5 may be a new strategy for a mucosal vaccine adjuvant against pneumococcal infection in the elderly. PMID: 26223660
  13. Over-activation of TLR5 signaling by high-dose flagellin induces liver injury in mice. PMID: 25418468
  14. results define systemically administered TLR5 agonists as organ-specific immunoadjuvants, enabling efficient antitumor vaccination that does not depend on identification of tumor-specific antigens PMID: 26831100
  15. an altered composition of the microbiota in a given environment can result in metabolic syndrome, but it is not a consequence of TLR5 deficiency per se PMID: 26950299
  16. aa 89-96 of flagellin is not only the crucial site responsible for TLR5 recognition, but is also important for humoral immune adjuvanticity through a TLR5-independent pathway. PMID: 25195514
  17. Gut inflammation in TLR5KO mice is dependent upon the presence of a gut microbiota. PMID: 26067589
  18. Tlr5 is co-expressed with neurofilament-200 in large-diameter A-fiber neurons in the dorsal root ganglion, skin nerve fibers, and spinal cord axonal terminals. PMID: 26479925
  19. These data show that TLR5 is a novel activator of RANKL and osteoclast formation and, therefore, a potential key factor in inflammation-induced bone erosions in diseases like RA, reactive arthritis, and periodontitis. PMID: 26207027
  20. gene deficiency results in an increase in susceptibility to invasive lung infection PMID: 25402425
  21. These results show that NLRC4 and TLR5, key components of two flagellin sensing pathways, each contribute to host defense in respiratory melioidosis. PMID: 25232720
  22. A new immunotherapeutic approach based on TLR5 activation and IFN-gamma production capable to control the metastatic growth of B16F10-Nex2 melanoma in mice. PMID: 25223833
  23. Data indicate that (131)I-radiolabelled anti-Toll-like receptor 5 (TLR5) monoclonal antibody (mAb) uptake in the grafts significantly correlated with TLR5 expression in the allograft area. PMID: 25283154
  24. TLR5 is important to limit myocardial damage, inflammation and functional compromise after myocardial ischemia-reperfusion. PMID: 25757463
  25. these findings indicate that the epithelial TLR5 participates in renal antibacterial defence, but paradoxically favours the translocation of Uropathogenic Escherichia coli across intact renal medullary collecting duct cell layers. PMID: 24779433
  26. Study demonstrates that TLR5 recognition of commensal microbiota regulates systemic tumor-promoting inflammation and, subsequently, extramucosal malignant progression. PMID: 25533336
  27. The results suggest a limited role for flagellin/TLR5 interactions in B. cereus endophthalmitis. PMID: 24959742
  28. Expression of TLR5 on intestinal epithelial cells regulates the composition and localization of the intestinal microbiota, preventing diseases associated with intestinal inflammation. PMID: 25172014
  29. these novel findings demonstrate that a direct and an indirect mechanism are involved in TLR5-driven rheumatoid arthritis inflammation and bone destruction. PMID: 25200955
  30. Airway structural cells regulate TLR5-mediated mucosal adjuvant activity. PMID: 24064672
  31. We discovered that TLR5, a cell surface receptor for bacterial protein flagellin, also requires UNC93B1 for plasma membrane localization and signaling. PMID: 24778236
  32. Data demonstrated that Notch1 synergistically increases TLR5mediated NF-kappaB activation. PMID: 24048326
  33. this study demonstrates IgG2c responses toward flagellin were TLR5 and inflammasome dependent; IgG1 was the dominant isotype and partially TLR5 and inflammasome dependent. PMID: 24442437
  34. These results indicate that polyethylenimine is a new TLR5 agonist with potential application in offering protection for patients receiving radiotherapy or in radiation-related accidents. PMID: 23104900
  35. These results suggest that cooperation of NOD and TLR receptors is important for effective responses to microbial infection in vivo. PMID: 23897616
  36. PTEN deletion impeded Mal localization at the plasma membrane and suppressed Mal-TLR5 interaction. These results suggest that, by controlling Mal recruitment, PTEN regulates TLR5-induced inflammatory responses. PMID: 23038756
  37. Cell surface expression of TLR5 is dependent on PRAT4A and restricted to neutrophils, classical monocytes and specific DC subsets. PMID: 22836022
  38. Both colitic and noncolitic TLR5-deficient mice exhibited transiently unstable microbiotas. PMID: 22863420
  39. a new beneficial effect of CBLB502, and suggests that TLR5-mediated immune modulation may be a promising approach to improve GVT immunity without exacerbating graft-versus-host disease. PMID: 23045613
  40. Mucosal dendritic cells (DCs), but not splenic DCs, express high levels of Tlr5 protein. PMID: 22545147
  41. TLR4 and TLR5 agonists lipopolysaccharide and flagellin, respectively, induced late degranulation mediated by TNF-alpha in Paneth cells. PMID: 21567398
  42. findings demonstrate that TLR5 engagement plays a major role in P. aeruginosa internalization and in triggering IL-1beta formation PMID: 22307620
  43. DUOX2 plays pivotal roles in TLR5-dependent inflammatory response of nasal airway epithelium. PMID: 21714724
  44. study found glucose up-regulates TLR-5 in islet cells, and that TLR-5 activation reduces insulin secretion, increases the level of heat-shock protein and increases MHC class I transport for presentation. PMID: 21985371
  45. study demonstrates that intestinal epithelia, despite not expressing IL-1beta, secrete sIL-1Ra in a TLR5-dependent manner suggesting that loss of TLR5 may promote inflammation by increasing IL-1beta activity PMID: 20844479
  46. We document a novel role for TLR5 in the rapid targeting of flagellin by intestinal pathogen-specific CD4 T cells PMID: 21451112
  47. TLR5 stimulation significantly improves pathological changes in the cecum and colon of Clostridium difficile-infected mice and reduces epithelial cell loss. PMID: 21245274
  48. An Myd88-independent function was uncovered for dendritic cell TLR5 in enhancing the presentation of peptides to flagellin-specific CD4+ T cells. PMID: 21182074
  49. the p110alpha and beta isoforms of class IA PI3K are both required for the proinflammatory response to flagellin via TLR5 PMID: 20953381
  50. TRIF deficiency inhibits TLR5-induced signaling in mouse intestinal epithelial cells. PMID: 20855887

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Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Toll-like receptor family
Tissue Specificity Highly expressed in liver. Detected in lung and at very low levels in most other tissues.
Database Links

KEGG: mmu:53791

STRING: 10090.ENSMUSP00000106625

UniGene: Mm.116894

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