Mouse Transforming protein RhoA(RHOA) ELISA kit

Instructions
Code CSB-EL019682MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name ras homolog gene family, member A
Alternative Names ARH12, ARHA, RHO12, RHOH12, Aplysia ras-related homolog 12|oncogene RHO H12|small GTP binding protein RhoA
Abbreviation RHOA
Uniprot No. Q9QUI0
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse RHOA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 94
Range % 89-98
1:2 Average % 87
Range % 83-92
1:4 Average % 92
Range % 87-98
1:8 Average % 93
Range % 86-97
Recovery
The recovery of mouse RHOA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 92-101
EDTA plasma (n=4) 89 86-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.145 2.249 2.197 2.045
500 1.376 1.472 1.424 1.272
250 0.875 0.925 0.900 0.748
125 0.557 0.541 0.549 0.397
62.5 0.386 0.392 0.389 0.237
31.2 0.295 0.301 0.298 0.146
15.6 0.212 0.219 0.216 0.064
0 0.151 0.153 0.152  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Required for the apical junction formation of keratinocyte cell-cell adhesion. Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis
Subcellular Location Cell membrane, Lipid-anchor, Cytoplasmic side, Cytoplasm, cytoskeleton, Cleavage furrow, Cytoplasm, cell cortex, Midbody, Cell projection, lamellipodium
Protein Families Small GTPase superfamily, Rho family
Database Links

KEGG: mmu:11848

STRING: 10090.ENSMUSP00000007959

UniGene: Mm.318359

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