Mouse Vesicular inhibitory amino acid transporter(SLC32A1) ELISA kit

Unavailable
Code CSB-EL021578MO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O35633
Lead Time 5-7 working days
Abbreviation SLC32A1
Protein Biological Process 1 Neurobiology
Target Name solute carrier family 32 (GABA vesicular transporter), member 1
Alias VGAT, VIAAT, GABA and glycine transporter|solute carrier family 32, member 1|vesicular GABA transporter|vesicular inhibitory amino acid transporter
Species Mus musculus (Mouse)
Protein Biological Process 3 Neurotransmitter transport
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 7.81 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SLC32A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 105
Range % 94-110
1:2 Average % 96
Range % 88-100
1:4 Average % 93
Range % 84-97
1:8 Average % 108
Range % 97-112
Recovery
The recovery of mouse SLC32A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 85-97
EDTA plasma (n=4) 101 92-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
500 2.161 2.222 2.192 2.033
250 1.523 1.500 1.512 1.353
125 1.072 1.039 1.056 0.897
62.5 0.786 0.767 0.777 0.618
31.25 0.549 0.536 0.543 0.384
15.62 0.390 0.402 0.396 0.237
7.81 0.267 0.259 0.263 0.104
0 0.157 0.161 0.159  
References
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Function Involved in the uptake of GABA and glycine into the synaptic vesicles.
Subcellular Location Cytoplasmic vesicle membrane, Multi-pass membrane protein
Protein Families Amino acid/polyamine transporter 2 family
Tissue Specificity Brain and retina. Localized in horizontal cell tips at both rod and cone terminals.
Database Links

KEGG: mmu:22348

STRING: 10090.ENSMUSP00000036299

UniGene: Mm.143404

Pathway Secreted Extracellular Vesicles

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