Mouse Xaa-Pro dipeptidase(PEPD) ELISA kit

Code CSB-EL017784MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name peptidase D
Alternative Names Pepd ELISA Kit; Pep4Xaa-Pro dipeptidase ELISA Kit; X-Pro dipeptidase ELISA Kit; EC ELISA Kit; Imidodipeptidase ELISA Kit; Peptidase 4 ELISA Kit; Peptidase D ELISA Kit; Proline dipeptidase ELISA Kit; Prolidase ELISA Kit
Abbreviation PEPD
Uniprot No. Q11136
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 mU/mL-1000 mU/mL
Sensitivity 3.9 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PEPD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %86
Range %82-90
1:2Average %94
Range %91-97
1:4Average %103
Range %100-106
1:8Average %99
Range %95-103
The recovery of mouse PEPD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9592-98
EDTA plasma (n=4)8986-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
10002.324 2.528 2.426 2.266
5001.578 1.455 1.517 1.357
2500.945 0.968 0.957 0.797
1250.586 0.574 0.580 0.420
62.50.345 0.349 0.347 0.187
31.20.250 0.259 0.255 0.095
15.60.194 0.198 0.196 0.036
00.156 0.163 0.160
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because of the high level of iminoacids in collagen.
Gene References into Functions
  1. The findings identify peptidase D, an ubiquitously expressed intracellular peptidase, as a potential novel marker of colitis. PMID: 28323866
  2. PEPD is a ligand of EGFR and presents a novel mechanism of EGFR activation PMID: 23212918
  3. The dark-like mutation causes cardiomyocyte hypertrophy due to loss-of-function of peptidase d (Pepd). PMID: 21472842
  4. NO stimulates prolidase activity by increasing serine/threonine phosphorylation through PKG-cGMP pathway PMID: 16167338
Protein Families Peptidase M24B family, Eukaryotic-type prolidase subfamily
Database Links

KEGG: mmu:18624

STRING: 10090.ENSMUSP00000075683

UniGene: Mm.69751


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