Mouse cluster of differentiation(CD44) ELISA Kit

Instructions
Code CSB-E13007m
Size 96T,5×96T,10×96T
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Product Details

Target Name CD44 molecule (Indian blood group)
Alternative Names Cd44 ELISA Kit; Ly-24CD44 antigen ELISA Kit; Extracellular matrix receptor III ELISA Kit; ECMR-III ELISA Kit; GP90 lymphocyte homing/adhesion receptor ELISA Kit; HUTCH-I ELISA Kit; Hermes antigen ELISA Kit; Hyaluronate receptor ELISA Kit; Lymphocyte antigen 24 ELISA Kit; Ly-24 ELISA Kit; Phagocytic glycoprotein 1 ELISA Kit; PGP-1 ELISA Kit; Phagocytic glycoprotein I ELISA Kit; PGP-I ELISA Kit; CD antigen CD44 ELISA Kit
Abbreviation CD44
Uniprot No. P15379
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD44 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %86
Range %80-92
1:2Average %94
Range %89-99
1:4Average %103
Range %99-107
1:8Average %99
Range %95-102
Recovery
The recovery of mouse CD44 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9592-99
EDTA plasma (n=4)8986-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.358 2.529 2.444 2.281
51.597 1.477 1.537 1.374
2.50.926 0.954 0.940 0.777
1.250.599 0.556 0.578 0.415
0.6250.331 0.333 0.332 0.169
0.3120.262 0.270 0.266 0.103
0.1560.198 0.204 0.201 0.038
00.157 0.169 0.163
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis (By similarity). Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function
Gene References into Functions
  1. this study shows that absence of specific alternatively spliced exon of CD44 in macrophages prevents colitis PMID: 29186109
  2. this study demonstrates a new role for CD44 and hyaluronan in promoting alveolar macrophage survival PMID: 29067996
  3. CD44-positive proliferating glomerular cells, most likely parietal epithelial cells, are essential in the pathogenesis of scarring glomerular disease. PMID: 29276101
  4. We show here that cleavage-stimuli-induced specific ICD-modifications cause structural substrate changes that enhance ectodomain sensitivity of neuregulin-1 (NRG1; epidermal-growth-factor) or CD44 (receptor-tyrosine-kinase (RTK) co-receptor) to chymotrypsin/trypsin or soluble ADAM. PMID: 27876763
  5. TGF-beta1-mediated hyaluronan/CD44/STAT3 signaling pathway plays a crucial role in the development of atrial fibrosis and atrial fibrillation. PMID: 28871329
  6. The role of CD44 was evaluated in CD44(-/-) challenged with a methionine- and choline-deficient diet (MCDD). Liver inflammation, liver injury and fibrosis strongly decreased in CD44(-/-) mice compared to wild-type mice on MCDD. PMID: 28323124
  7. Data (including data from studies using transgenic mice) suggest that a single oral dose of vitamin A (1) amplifies tolerogenic activity of dendritic cells migrating to lymphoid tissue and (2) up-regulates expression of Foxp3 and Cd44 in co-cultures of dendritic cells and CD4+ lymphocytes. (Foxp3 = forkhead box P3; Cd44 = homing receptor) PMID: 28917953
  8. The pseudo-hypoxic phenotype of stem-like glioma cells is achieved by stabilization of HIF-2a through interaction with CD44, independently of oxygen. PMID: 28813675
  9. the survival outcomes of genetically induced glioma-bearing mice and of high-grade human glioma patients are biphasically correlated with CD44 level, with the poorest outcomes occurring at intermediate levels. PMID: 28052252
  10. pERK1/2 is a regulator of CD44 expression, and increased CD44 expression leads to a pro-sclerotic and migratory parietal epithelial cell phenotype in focal segmental glomerulosclerosis. PMID: 27998643
  11. MET signaling regulates intestinal homeostasis and regeneration, as well as adenoma formation. These activities of MET are promoted by the stem cell CD44 isoform CD44v4-10. PMID: 28716720
  12. study demonstrates that CD44 plays a critical role in the regulation of experimental autoimmune encephalomyelitis through alterations in the gut microbiome profile and short-chain fatty acid production PMID: 28543188
  13. CD44 variant isoform 9 emerges in response to injury and contributes to the regeneration of the gastric epithelium. PMID: 28497484
  14. OA/GPNMB acts through ERK and AKT signaling pathways in mesenchymal stem cells via CD44 PMID: 26442636
  15. These findings support the usefulness of the conditional Cd44 allele in unraveling essential physiological and pathological functions of CD44 isoforms. PMID: 27831556
  16. CD44 role in the atrophic epidermis PMID: 28099467
  17. CD44(high) type II cells likely represent a type II cell subpopulation important for constitutive regulation of alveolar homeostasis. PMID: 28473330
  18. CD44V6 is part of a positive-feedback loop with TGFbeta1/TGFbetaRI signaling that acts to increase NOX4/ROS production, which is required for myofibroblast differentiation, myofibroblast differentiation, myofibroblast extracellular matrix production, myofibroblast invasion, and myofibroblast contractility. PMID: 28389561
  19. HAS2-produced hyaluronan is required for CD44V6 and TGFbetaRI co-localization and subsequent CD44V6/ERK1/EGR1 signaling. These results demonstrate a novel positive-feedback loop that links the myofibroblast phenotype to TGFbeta1-stimulated CD44V6/ERK/EGR1 signaling. PMID: 28389562
  20. CD44 promotes accumulation of M2 macrophages, extracellular matrix deposition, and wall thickening during arteriovenous fistula maturation. PMID: 28450292
  21. CD44-expressing Neural Stem Cells are self-renewing and multipotent and they synthesize Hyaluronan that accumulates with aging. CD44-null Neural Stem Cells demonstrate increased proliferation in vitro and in vivo. PMID: 28154169
  22. CD44 knockout mice are more susceptible to stress-induced anxiety-like behavior than control mice. PMID: 27619521
  23. CD44 contributes to effective antibacterial defense during S. zooepidemicus infection, thereby limiting the accompanying injury and death. PMID: 28062002
  24. These studies demonstrate that both endometrial cell and peritoneal mesothelial cell CD44 play a role in the development of early endometriotic lesion. PMID: 26169039
  25. Targeting CD44 expressing colon cancer cells with anti-CD44 monoclonal antibody improves cellular uptake and antitumor efficacy of liposomal doxorubicin. PMID: 26518722
  26. depletion of CD44s inhibits breast cancer cell metastasis to the lung in animals. These findings suggest a crucial mechanism underlying the role of the CD44s splice isoform in breast cancer metastasis. PMID: 26869223
  27. MGK as hydrophobic moieties and oHA as the target of CD44 receptor. PMID: 24892632
  28. CD44, NOD2, and Fas have distinct functions in regulating changes in trabecular and cortical bone indices during unloading. PMID: 26546009
  29. The absence of CD44 in neonatal bone marrow has no impact on the size of the long-term reconstituting hematopoietic stem cell pool, but results in an enhanced long-term engraftment potential of hematopoietic stem cells. PMID: 26546504
  30. CD44 deficiency can reduce osteoclast activity and counteract cortical bone loss in the hindlimb of unloaded mice. PMID: 26530337
  31. Genetic deficiency of CD44 augments activation, apoptosis and pro-thrombotic potential of platelets. PMID: 26355696
  32. The high targeting ability of HA-MFNPs was observed at MKN-45 cells (gastric cancer cell line) which high-expressing CD44 in contrast with MKN-28 cells which low-expressing CD44. PMID: 27398445
  33. the aggravation of pulmonary inflammation upon combined exposure to allergen and an environmental pollutant is CD44-dependent. PMID: 26999446
  34. CD44 promotes chemoresistance in T-ALL by increased drug efflux. PMID: 26708679
  35. HA-binding to CD44-expressing beta cells is implicated in beta-cell demise PMID: 26624007
  36. Peptide inhibitors of CD44v6 isoforms block tumor growth and metastasis in several independent models of pancreatic cancer. PMID: 26597578
  37. Suggest CD44 is involved in the development of mucous metaplasia in mice infected with Helicobacter pylori. PMID: 26639196
  38. CD44 enhances T cell receptor signaling strength by delivering lymphocyte-specific protein kinase, which is required for induction of IL-17 producing T helper cells PMID: 26172046
  39. Endogenous hyaluronic acid increases Lgr5(+) stem cell proliferation, crypt fission, and intestinal lengthening and that these effects are dependent on signaling through CD44 and TLR4. PMID: 26505972
  40. consistent with CD44's pivotal role in modulating several diverse behaviors important for adhesion, proliferation, apoptosis, migration and invasion during development, growth, repair, maintenance and regression of a wide variety of mesenchymal tissues PMID: 26248063
  41. The current study reports a unique approach to study H. pylori interaction with the human gastric epithelium. Here, we show that CD44 plays a functional role in H. pylori-induced epithelial cell proliferation. PMID: 25658601
  42. CD44 and pro-NRG1 are posttranslationally modified at their intracellular domains. PMID: 26217011
  43. Data indicate that a short course of anti-CD44 treatment improved Treg cell therapy efficacy in type 1 diabetes (T1D) model. PMID: 25447401
  44. Thymic innate CD8(+)CD44(hi) SP T-cells have critical roles in influenza A(H1N1)pdm09 infection-induced thymic atrophy through secreting IFN-gamma. PMID: 25275588
  45. CD44 deficiency could result in decreased proinflammatory cytokine production in lung induced by porcine circovirus type 2 (PCV2) in mice, suggesting a previously unrecognized role for CD44 in the development of pneumonia response to PCV2 infection. PMID: 25294252
  46. PRG4 is a novel putative ligand for CD44 and may control synoviocyte overgrowth in inflammatory arthropathies via a CD44-mediated mechanism. PMID: 25708025
  47. These data support an important role for CD44 in locomotor and sensorimotor functions, and in spatial memory retention. PMID: 25219362
  48. This study displays for the first time a different role of CD44-specific isoforms in the progression of chronic kidney disease. PMID: 24717295
  49. We hypothesize that polyvalent electrostatic interactions are responsible for the assembly of CD44 clusters and the multimeric PIP2-CD44-Ezrin complexes. PMID: 25572402
  50. of the transcripts of tissue inhibitors of matrix metalloproteinases (TIMPs) showed that expression of both TIMP1 and TIMP2 correlates negatively with the invasive potential of cells. PMID: 25180193
  51. Data show that actin cytoskeleton regulated CD44 membrane mobility and interactions with E-selectin. PMID: 25359776
  52. regulatory factor 1 directly regulates CD44 expression in glioblastoma PMID: 24526308
  53. The perturbation of CD82-ganglioside-CD44 signaling attenuates pathological angiogenesis. PMID: 25149363
  54. These results indicate that parasite-invaded leukocytes gain higher ability to adhere to hyaluronan than non-invaded leukocytes, via upregulation of CD44 expression and/or selective invasion to CD44 highly expressing cells. PMID: 24157443
  55. The CD44 ligand osteopontin shared a perivascular expression pattern with CD44 and promoted glioma stem cell-like phenotypes. PMID: 24607407
  56. Lack of CD44 impairs the early pro-inflammatory cytokine response to lipopolysaccharides, diminishes leukocyte migration/chemotaxis and endothelial activation, hence, delays endotoxic shock-induced acute kidney injury. PMID: 24376813
  57. Rb suppresses collective invasion, circulation and metastasis of breast cancer cells in CD44-dependent manner. PMID: 24324613
  58. osteopontin, acting via CD44 receptors, induces apoptosis in myocytes via the involvement of mitochondrial death pathway and endoplasmic reticulum stress PMID: 24531809
  59. SP/drug efflux functionality of hematopoietic progenitors is controlled by mesenchymal niche through VLA-4/CD44 axis. PMID: 23999380
  60. c-Met and CD44v6 receptors have roles in autocrine TGF-beta1 signaling in interstitial lung disease PMID: 24324260
  61. CD44 deficiency is accompanied by reduction in hyaluronan staining in CD44 knock out skin leading to a marked thinning of epidermis vs. wildtype skin PMID: 23790635
  62. CD44 regulation of endothelial cell proliferation and apoptosis via modulation of CD31 and VE-cadherin expression. PMID: 24425872
  63. the CD44v isoform, but not CD44s, promotes adenoma initiation in Apc(Min/+)mice. Our data identify CD44v as component of the ISCs program critical for tumor initiation, and as potential treatment target in CRC. PMID: 23318432
  64. CD44 and MT1-MMP are directly or indirectly involved in the regulation of pro-MMP9 activation. PMID: 23984338
  65. CXCL12-induced CXCR4 signaling depends on the binding of high-molecular-weight hyaluronic acid to CD44. PMID: 24091662
  66. At the initial stage of the endotoxemia, leukocyte sequestration depends on CD44 but is independent of ICAM-1 and beta2-integrins. PMID: 23726757
  67. absence of CCR2 completely and CD44 partially reduces hepatic leukocyte recruitment. PMID: 23762326
  68. these results reveal an unrecognized role for CD44 in oncogenic Kras-induced lung adenocarcinoma and suggest that targeting CD44 could be an effective strategy for halting Kras-dependent carcinomas. PMID: 23208496
  69. Mesenchymal CD44 expression contributes to the acquisition of an activated fibroblast phenotype via TWIST activation in the tumor microenvironment. PMID: 23838935
  70. CD44v was expressed de novo in spasmolytic polypeptide-expressing metaplasia in mouse models of gastric carcinogenesis. PMID: 23848514
  71. CD44 exerts a protective effect on vascular barrier integrity, and does so by a PECAM-1-dependent mechanism. PMID: 23504212
  72. These in vitro and in vivo data suggest that CD44 may be crucial not only for NPC crossing the endothelial layer but also for facilitating invasion of extravascular tissues PMID: 23468987
  73. CD44 plays a critical role in regulating several aspects of metabolic syndrome and may provide a new therapeutic target in the management of insulin resistance. PMID: 23505504
  74. Report CD44/macrophage mannose receptor as a ligand/receptor pair involved in the process of lymphocyte transit via the lymphatic vasculature. PMID: 23603511
  75. CD44 has multiple protective roles in experimental autoimmune encephalomyelitis. PMID: 23416161
  76. an in vivo signaling pathway, ERK --> CD44 --> STAT3, that regulates normal and atrophy-induced gastric stem/progenitor-cell proliferation. PMID: 23589310
  77. in mouse embryonic fibroblasts, reprogramming follows an orderly sequence of stage transitions, marked by changes in the cell-surface markers CD44 and ICAM1 PMID: 23728301
  78. CD44 Promotes intoxication by the clostridial iota-family toxins. PMID: 23236484
  79. Loss of CD44 attenuates aberrant GM-CSF signaling in Kras G12D-induced hematopoietic malignancies. PMID: 22976127
  80. Hyaluronan anchored to activated CD44 on central nervous system vascular endothelial cells promotes lymphocyte extravasation in experimental autoimmune encephalomyelitis PMID: 22865853
  81. In the developing cerebellum, CD44-positive cells, mostly located in the white matter, are positive for markers of the astrocyte lineage, but negative for the markers of mature astrocytes. PMID: 21732075
  82. OPN plays a significant role in the proliferation of hepatocellular carcinoma through interaction with the cell surface receptor CD44 PMID: 22807608
  83. CD44 retains hyaluronan in the keratinocyte pericellular matrix, a fact that has not been shown unambiguously before, and that hyaluronan abundance in the absence of CD44 can result in hyaluronan trapping in abnormal locations possibly interfering there with normal differentiation and epidermal barrier function PMID: 22072421
  84. CD44 expression in astrocytes and microglia is closely associated with the pathogenesis of ALS, and suggests that inflammatory responses involving CD44 may play a role in this disease. PMID: 22634473
  85. CD44, as expressed by LS174T colon carcinoma cells, functions as a tumor suppressor. When the expression of CD44 is silenced, we see a dramatic increase in metastatic potential PMID: 22415308
  86. Cross-linking of macrophage CD44 is associated with increased phagocytosis of apoptotic polymorphonuclear leukocytes. PMID: 22427969
  87. These results suggest the possibility of targeting CD44 for the treatment of staphylococcal enterotoxin B-induced acute lung injury/acute respiratory distress syndrome PMID: 22659034
  88. activation of P2X7R by ATP induces CD44 shedding. PMID: 21812012
  89. Results suggest that the CD44 and RHAMM receptors function on membrane lipid rafts during Cryptococcus neoformans invasion. PMID: 22418440
  90. CD44 deficiency in a diabetic model ameliorates insulin resistance and adipose tissue inflammation, and anti-CD44 antibody treatment decreases blood glucose levels and adipose tissue macrophage accumulation in a high-fat diet-fed model. PMID: 22499789
  91. Data show that adoptively transferred CD44 wildtype lymphokine-activated killer (LAK) cells were significantly more effective than CD44 knockout LAK cells at controlling tumor growth in vivo. PMID: 21901391
  92. Data indicate a plausible Plag1-Egr1-CD44 regulatory pathway in the T-ICs of pleomorphic adenomas. PMID: 21858056
  93. CD44 expressed on CD4(+) T cells plays a critical role in the accumulation of antigen-specific Th2 cells, but not Th1 cells, in the airway and in the development of airway hyperresponsiveness PMID: 21874648
  94. ln epidermal keratinocytes, ultraviolet radiation increased hyaluronic acid and CD44 antigen levels in premalignant hyperplasia, dysplasia and squamous cell carcinoma. PMID: 21832148
  95. signaling through CD44, in encephalitogenic T cells, plays a crucial role in the differentiation of Th cells through epigenetic regulation, specifically DNA methylation of Th1/Th17 and Th2 cytokine genes PMID: 21551360
  96. role of ADAMTS5 in dermal repair through CD44-mediated aggrecan accumulation and modulation of transforming growth factor beta1 (TGFbeta1) signaling PMID: 21566131
  97. Both the invasive phenotype and the progressive severe bleomycin-induced pulmonary fibrosis were inhibited in the absence of CD44. PMID: 21708929
  98. We further show that CD44 plays a role in drug resistance, independently of P-gp. PMID: 21357442
  99. Our results identify additional mechanisms by which CD44 modulates the inflammatory response PMID: 21457936
  100. Differential use of chondroitin sulfate to regulate hyaluronan binding by receptor CD44 in Inflammatory and Interleukin 4-activated Macrophages. PMID: 21471214

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Subcellular Location Cell membrane, Single-pass type I membrane protein
Database Links

KEGG: mmu:12505

STRING: 10090.ENSMUSP00000005218

UniGene: Mm.423621

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