Mouse coagulation factor Ⅱ,FⅡ ELISA Kit

Instructions
Code CSB-E08631m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name coagulation factor II (thrombin)
Alternative Names F2 ELISA Kit; Cf2 ELISA Kit; Prothrombin ELISA Kit; EC 3.4.21.5 ELISA Kit; Coagulation factor II) [Cleaved into: Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] ELISA Kit
Abbreviation F2
Uniprot No. P19221
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 0.156 μg/mL-10 μg/mL
Sensitivity 0.039 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅡ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 97
Range % 93-102
1:200 Average % 86
Range % 81-92
1:400 Average % 102
Range % 97-108
1:800 Average % 92
Range % 88-96
Recovery
The recovery of mouse FⅡ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 91-100
EDTA plasma (n=4) 101 97-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
µg/ml OD1 OD2 Average Corrected
10 2.254 2.143 2.199 2.072
5 1.894 1.812 1.853 1.726
2.5 1.376 1.342 1.359 1.232
1.25 0.816 0.796 0.806 0.679
0.625 0.432 0.415 0.424 0.297
0.312 0.286 0.280 0.283 0.156
0.156 0.198 0.195 0.197 0.070
0 0.128 0.126 0.127  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity).
Gene References into Functions
  1. These results suggested that the thrombinstimulated synthesis of IL6 was limited by HSP90 in osteoblasts, and that the effects of HSP90 were exerted at the point between Rhokinase and p38 MAPK. PMID: 30066835
  2. Platelet derived microparticles formed in sepsis are a potent inducer of thrombin generation via phosphatidylserine exposure, and activation of both the intrinsic and extrinsic pathway of coagulation. PMID: 28409836
  3. These findings imply that a thrombin-pericyte interaction in the brain could be a significant mechanism responsible for blood-brain barrier dysfunction under obesity-associated diabetic conditions and thus could represent a therapeutic target for central nervous system complications of diabetes. PMID: 28489922
  4. mutations were introduced into the endogenous fII gene, resulting in expression of prothrombin carrying 3 amino acid substitutions (R157A, R268A, and K281A) to limit activation events to yield only fIIa(Meizothrombin) Homozygous fII(MZ) mice are viable, express fII levels comparable with fII(Wild type) mice, and have reproductive success. PMID: 27252233
  5. allosteric inhibitor SbO4L targets the glycoprotein Ibalpha-binding and heparin-binding site of thrombin PMID: 26748875
  6. thrombin/PAR-1 interaction regulated MCP-1, TF, MCSF and IL-6 production. PMID: 26475502
  7. Observations illustrate the role of thrombin as a pleiotropic atherogenic molecule under conditions of hypercholesterolemia and suggest the utility of its inhibition with locally acting antithrombin nanoparticle therapeutics. PMID: 26769047
  8. Thrombin-PAR1 signaling, via nitric oxide and EPCR, promotes hematopoietic stem cell (HSC) mobilization. PMID: 26457757
  9. Primary tumor growth by colon cancer cells was reduced by reduction of circulating prothrombin. There were lower mitotic indices and invasiveness. This growth relied upon 2 targets of thrombin-mediated proteolysis, protease: PAR-1 and fibrinogen. PMID: 26238780
  10. Thrombin promotes sustained signaling and inflammatory gene expression through the CDC25 and Ras-associating domains of phospholipase C epsilon. PMID: 26350460
  11. thrombin/PAR1 pathway as a novel target for developing therapeutic strategies to restore synaptic function in the acute phase of ischemic stroke. PMID: 25604482
  12. High Factor IIa expression is associated with multiorgan pathologies in sickle cell disease. PMID: 26286849
  13. Blockage of thrombin exosites with compounds specific for exosite I (hirudin and HD1 aptamer) or exosite II (heparin and HD22 aptamer) impaired the COMP-thrombin interaction, indicating a 2-site binding mechanism. PMID: 26045608
  14. Fyn, but not Lyn, was required for complete Pyk2 phosphorylation by thrombin. PMID: 25967238
  15. Thrombin cleavage of osteopontin disrupts a pro-chemotactic sequence for dendritic cells, which is compensated by the release of its pro-chemotactic C-terminal fragment. PMID: 25112870
  16. The Ras-related protein, Rap1A, mediates thrombin-stimulated, integrin-dependent glioblastoma cell proliferation and tumor growth. PMID: 24790104
  17. Influenza virus H1N1 activates platelets through FcgammaRIIA signaling and thrombin generation. PMID: 24665136
  18. These results indicate that thrombin acts through multiple mechanisms to activate MMPs and PGE2 synthesis in amnion. PMID: 24652285
  19. Suggest a thrombin-dependent mechanism of complement activation by red blood cell-microvesicles independent of the classical, lectin, or alternative pathway. PMID: 24311376
  20. thrombin induces monocyte/macrophage migration via PAR1-Galpha12-dependent Pyk2-mediated Gab1 and p115 RhoGEF interactions, leading to Rac1- and RhoA-targeted Pak2 activation. PMID: 24025335
  21. Micro-computed tomography analysis of distal tibia metaphyses also revealed for the first time a major impact of the FVIII/thrombin/PAR1 axis on the dynamic bone structure. PMID: 23982175
  22. PKCalpha and Akt modulate platelet function by phosphorylating and inhibiting GSK3alpha/beta, thereby relieving the negative effect of GSK3alpha/beta on thrombin-mediated platelet activation. PMID: 23239877
  23. Mast cell restricted mouse and human tryptase.heparin complexes hinder thrombin-induced coagulation of plasma and the generation of fibrin by proteolytically destroying fibrinogen. PMID: 22235124
  24. Recombinant thrombomodulin domains 2 and 3 protein significantly reduces atherosclerosis and neointima formation through its thrombin-binding ability. PMID: 21840881
  25. Protective roles for fibrin, tissue factor, plasminogen activator inhibitor-1, and thrombin activatable fibrinolysis inhibitor, but not factor XI, during defense against the gram-negative bacterium Yersinia enterocolitica. PMID: 21724997
  26. Active site-labeled prothrombin inhibits prothrombinase in vitro and thrombosis in vivo PMID: 21531712
  27. The development of inflammatory joint disease is attenuated in mice expressing the anticoagulant prothrombin mutant W215A/E217A. PMID: 21436072
  28. Localization and expression of prothrombin in rodent osteoclasts and long bones. PMID: 21191574
  29. platelet STIM1 and Orai1 have roles in glycoprotein VI- and thrombin-dependent procoagulant activity and thrombus formation PMID: 20519511
  30. Hirudin preconditioning of vWF-deficient mice attenuated processing of infused rpvWF suggesting that thrombin plays a part in the processing events in vivo. PMID: 11776313
  31. effect of hirudin treatment indicates endogenous thrombin contributes to tumor implantation, seeding, and spontaneous metastasis PMID: 15265791
  32. mouse thrombin lacks Na+ activation but retains high catalytic activity PMID: 16428384
  33. beta(3) integrin, thrombin, and Ca(2+) play critical roles in platelet aggregation without fibrinogen or VWF PMID: 16824188
  34. Thrombin and MMP-9 collaborate to promote neuronal death in culture and in intracerebral hemorrhage (ICH). PMID: 17021183
  35. findings indicate thrombin plays a major role in extraintestinal thrombus formation associated with experimental colitis PMID: 18772359
  36. thrombin-induced p65/RelA, AMPK, and PKCdelta activation were markedly reduced by knockdown of the TRPC isoform TRPC1 expressed in human endothelial cells and in endothelial cells obtained from Trpc4 knock-out mice. PMID: 18990707
  37. formation of thrombin through the procoagulant activity of FVIII is necessary to induce costimulation for the immune response to FVIII treatment PMID: 19794141

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Protein Families Peptidase S1 family
Database Links

KEGG: mmu:14061

STRING: 10090.ENSMUSP00000028681

UniGene: Mm.89048

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