Mouse coagulation factor VIIIa light chain (F8aLC) ELISA kit

Code CSB-EQ027659MO
See More Details 24T ELISA kits trial application
Product Type ELISA kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation F8aLC
Target Name coagulation factor VIIIa light chain (F8aLC)
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse F8aLC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 106
Range % 103-109
1:200 Average % 87
Range % 83-90
1:400 Average % 107
Range % 103-110
1:800 Average % 96
Range % 93-99
Recovery
The recovery of mouse F8aLC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 108 104-112
EDTA plasma (n=4) 92 89-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
100 0.099 0.098 0.099
50 0.204 0.210 0.207
25 0.349 0.329 0.339
12.5 0.504 0.512 0.508
6.25 0.766 0.772 0.769
3.12 1.006 1.063 1.035
1.56 1.350 1.400 1.375
0 2.167 2.200 2.184
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