Mouse hepatic lipase,HL ELISA Kit

Code CSB-E10033m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name lipase, hepatic
Alternative Names Lipc ELISA Kit; Hpl ELISA Kit; Hepatic triacylglycerol lipase ELISA Kit; HL ELISA Kit; Hepatic lipase ELISA Kit; EC 3.1.1.3 ELISA Kit; Lipase member C ELISA Kit
Abbreviation LIPC
Uniprot No. P27656
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates
Detection Range 0.625 U/mL-40 U/mL
Sensitivity 0.156 U/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 103
Range % 95-107
1:2 Average % 96
Range % 87-99
1:4 Average % 92
Range % 83-95
1:8 Average % 105
Range % 94-109
Recovery
The recovery of mouse HL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 91-105
EDTA plasma (n=4) 98 84-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/ml OD1 OD2 Average Corrected
40 2.874 2.811 2.843 2.679
20 2.575 2.478 2.527 2.363
10 2.096 2.035 2.066 1.902
5 1.449 1.431 1.440 1.276
2.5 0.796 0.823 0.810 0.646
1.25 0.523 0.532 0.528 0.364
0.625 0.306 0.314 0.310 0.146
0 0.166 0.162 0.164  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin.
Gene References into Functions
  1. The LIGHT (Tumour necrosis factor ligand superfamily member 14, TNFSF14)/Lymphotoxin beta-Receptor(LTbeta-R) pathway, which is involved in T-cell and macrophage activation, was diminished in plasma and in apoE-/-Irs2+/-HL-/- atheromas. PMID: 27172975
  2. hepatic lipase KO mice had dyslipidemia including hypercholesterolemia, hypertriglyceridemia and increased non-esterified fatty acid levels. Also glucose intolerance, pancreatic and hepatic inflammation and steatosis. PMID: 26423094
  3. Bone marrow (BM)-derived hepatic lipase mitigates the HDL-lowering, HDL-modulating, and cholesterol-raising effects of BM-derived cholesteryl ester transfer protein. PMID: 24818611
  4. HL expressed by osteoblasts has an impact on osteoblast OPG expression and that lack of HL leads to increased bone mass. PMID: 24440515
  5. Hepatic lipase and endothelial lipase influence molecular species of several classes of plasma lipids. PMID: 24777581
  6. interaction of LIGHT with LTbetaR on hepatocytes, but not Kupffer cells, is sufficient to down regulate hepatic lipase expression and that this effect can be independent of LIGHT's costimulatory function. PMID: 23355893
  7. Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties. PMID: 23328279
  8. Data show a novel pathway involving HL hydrolysis of VLDL that activates PPARdelta through generation of specific monounsaturated fatty acids. PMID: 21750705
  9. These results demonstrated that fatty acid synthase and hepatic lipase might be FXR-regulated genes in liver cells. PMID: 20373033
  10. The additive effect of hepatic lipase and endothelial lipase on HDL metabolism but not macrophage reverse cholesterol transport in mice. PMID: 20558822
  11. highly divergent effects of naturally occurring HL coding sequence variants on lipid and lipoprotein metabolism PMID: 19965617
  12. HL deficiency protects against diet-induced obesity and its hepatic sequelae. PMID: 20056822
  13. Data show that shows the arrangement of an evolutionarily conserved domain within LMF1 (DUF1222) that is essential to lipase maturation. PMID: 19783858
  14. findings indicate that hepatic lipase is required for optimal reproductive performance PMID: 11906928
  15. synthesized in peritoneal macrophages PMID: 11971936
  16. indicate a role for hepatic lipase in clearing phospholipase/free cholesterol from the vesicular lipoproteins of phospholipid transfer protein knock out mice. PMID: 12117557
  17. the determinants of cell surface binding exist within the carboxyl terminal 70 amino acids of hepatic lipase, of which the last five residues play an important role PMID: 12700335
  18. Hepatic lipase expression in macrophages contributes to atherosclerosis in apoE-deficient and LCAT-transgenic mice. PMID: 12897204
  19. Not essential for diet-induced gallstone formation in mice. PMID: 12939599
  20. hepatic lipase activity is influenced by epistatic interaction between two nonstructural loci on chromosomes 7 and 3 PMID: 15314098
  21. The results demonstrate that hepatic lipase deficiency, like LDLR deficiency, blunts the adrenal response to chronic ACTH stimulation and suggest a novel role for hepatic lipase in adrenal physiology. PMID: 16368783
  22. HL-deficiency delays the onset and/or progression of atherosclerosis via a SR-BI-independent mechanism PMID: 16397139
  23. Establishment of an antiatherogenic role of the ligand-binding function of hepataic lipase in LDL receptor-deficient mice. PMID: 17071916
  24. ciHL expression reduces atherosclerosis PMID: 17234195
  25. Elevated PLTP activity lowers plasma HDL-cholesterol by stimulating the plasma turnover and hepatic uptake of HDL cholesteryl esters. PMID: 19524061

Show More

Hide All

Subcellular Location Secreted
Protein Families AB hydrolase superfamily, Lipase family
Database Links

KEGG: mmu:15450

STRING: 10090.ENSMUSP00000034731

UniGene: Mm.390187

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1