Mouse hypoxia-inducible factor 1α,HIF-1α ELISA Kit

Code CSB-E08541m
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details


This Mouse HIF1A ELISA Kit was designed for the quantitative measurement of Mouse HIF1A protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.625 pg/mL-40 pg/mL and the sensitivity is 0.156 pg/mL .

Target Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Alternative Names Hif1aHypoxia-inducible factor 1-alpha ELISA Kit; HIF-1-alpha ELISA Kit; HIF1-alpha ELISA Kit; ARNT-interacting protein ELISA Kit
Abbreviation HIF1A
Uniprot No. Q61221
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 0.625 pg/mL-40 pg/mL
Sensitivity 0.156 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HIF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 100  
Range % 95-107  
1:200 Average % 89  
Range % 83-98  
1:400 Average % 91  
Range % 87-95  
1:800 Average % 96  
Range % 90-101  
The recovery of mouse HIF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-95  
EDTA plasma (n=4) 94 90-98  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
40 2.253 2.233 2.243 2.186  
20 1.346 1.275 1.311 1.254  
10 0.792 0.752 0.772 0.715  
5 0.468 0.491 0.480 0.423  
2.5 0.263 0.264 0.264 0.207  
1.25 0.222 0.240 0.231 0.174  
0.625 0.118 0.122 0.120 0.063  
0 0.055 0.058 0.057    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
Gene References into Functions
  1. MiR-497 approximately 195 cluster regulates angiogenesis during coupling with osteogenesis by maintaining endothelial Notch1 and HIF1A activity. PMID: 28685750
  2. HIF-1alpha activation in myeloid cells critically regulates Inflammatory bowel disease progression. PMID: 29967068
  3. HOXA9 inhibits HIF1A-mediated glycolysis through interacting with CRIP2 to repress cutaneous squamous cell carcinoma development. PMID: 29662084
  4. Overexpression of HIF1A leads to radioresistance of cervical cancer. PMID: 30355300
  5. Studies demonstrate a role for HIF-1alpha in regulation of TSP2 expression via primarily a transcriptional mechanism. PMID: 28789925
  6. HIF1alpha role in the cardiac hypertrophy, apoptosis, and fibrosis.HIF1alpha is partially inhibited in the cardiac fibrosis by p53. PMID: 29729274
  7. T3 thyroid hormone increased HIF-1alpha protein levels as well as steroid secretion in Leydig cells PMID: 29417848
  8. Myeloid-specific deletion of Hif1a had no impact on the number of myeloid cells migrating into the eye. PMID: 28112274
  9. HS-induced PPARalpha-mediated downregulation of PHD1 is a novel pathway for PHD/HIF-1alpha transcriptional regulation. PMID: 29500923
  10. HIF1alpha promoted macrophage glycolysis metabolism, which induced M1 polarization in mice. PMID: 29386753
  11. DAPK deficiency leads to excess HIF-1a accumulation, enhanced IL-17 expression and exacerbated experimental autoimmune encephalomyelitis. PMID: 27312851
  12. activation of HIF signaling in osteoprogenitor cells increases blood levels of the chemokine C-X-C motif ligand 12, which leads to a systemic increase of breast cancer cell proliferation and dissemination through direct activation of the CXCR4 receptor PMID: 29339479
  13. HIF regulation of HOIL-1L targets the phosphorylated PKC-zeta for degradation and serves as an hypoxia-adaptive mechanism to stabilize the Na,K-ATPase, avoiding significant lung injury. PMID: 29109255
  14. our study suggests that HIF1A is a common critical downstream mediator for HLH. We propose that HIF1A activation as the consequence of systemic inflammation, cytokine storm, or ligation of TLR may contribute to HLH development. PMID: 28860338
  15. Decreased HIF-1alpha may contribute to impaired megakaryopoiesis in immune thrombocytopenia. PMID: 28771276
  16. The results collectively suggest that in the pressure overload heart failure model, HSF1 promoted formation of macrophages by inducing upregulation of HIF-1 expression, through which heart failure was ameliorated. PMID: 29678661
  17. Hypoxia-inducible factor 1 alpha deficiency is associated with Renal injury in a mouse model of STZ-induced diabetes. PMID: 28774305
  18. Results showed that Mn-mediated age-related hearing loss involved an increased level of Hif-1alpha protein with less hydroxylation at proline 564 in spiral ganglion neurons. PMID: 27824154
  19. Aneurysmal-prone factors induced HIF-1alpha can cause overexpression of MMP-2 and MMP-9 and promote aneurysmal progression. PMID: 27363580
  20. endothelial progenitor cell protected blood-brain barrier integrity after focal ischemia in diabetic mice through upregulation of HIF-1alpha signaling. PMID: 28697748
  21. HIF-1 dependent profibrogenic mechanisms are operating at the early onset of renal fibrosis but its contribution declines with the progression in mouse unilateral ureteral obstruction model. PMID: 29352658
  22. HIF1A dependent wound healing angiogenesis in vivo can be controlled by site-specific lentiviral magnetic targeting of SHP2. PMID: 28434868
  23. Study shows for the first time that mitochondrial defects which are not related to the electron transport chain and Krebs cycle enzymes contribute differently to HIF-1alpha regulation by affecting HIF-1alpha degradation and HIF-1alpha transcription with reactive oxygen species being rather indirectly involved. PMID: 28531964
  24. Driving IL-10 expression in CD1d(hi)CD5(+) B cells is a key function of the hypoxia-associated transcription factor HIF-1alpha. PMID: 29343683
  25. Benzopyranyl 1,2,3-triazole compound inhibits HIF-1alpha by accelerating its degradation, and shows much potential as an anti-cancer agent through its ability to suppress tumor growth and angiogenesis. PMID: 27999195
  26. that HIF-1 plays an important role in hyperglycemia-induced exacerbation of BBB disruption in ischemic stroke PMID: 27425889
  27. HIF-1alpha/miR-210 pathway is strongly activated in VHL mutated paragangliomas, weakly activated in SDHx mutated PGLs, and not activated in VHLdel- and SDHxwt/VHLwt-PGLs. PMID: 28036268
  28. Data indicate the important role of hypoxia inducible factor-1 (HIF-1) in the initial progression of immobilization-induced joint contracture, suggesting the possibility of molecular treatment to prevent the progression of joint contracture prior to intervention with physical therapy. PMID: 27352194
  29. HIF1alpha-regulated profile defines a melanocyte-specific response under hypoxia, and demonstrates the role of HIF1alpha as an invasive cell state gatekeeper in regulating cellular metabolism, chromatin and transcriptional regulation, vascularization, and invasion. PMID: 28168807
  30. omega-alkynyl arachidonic acid may promote the anti-inflammatory M2 polarization of macrophages in acute myocardial infarction via regulating the cross-talk between PKM2, HIF-1alpha and iNOS. PMID: 28964797
  31. Herein we report that TGM1 is a HIF-1-regulated gene that is associated with the upregulation of airway epithelial junction proteins, supporting a protective role for HIF-1 in the lung. PMID: 27423780
  32. the role of hypoxia-inducible factor-1 for myeloid cell function and immune response during colitis, was investigated. PMID: 29261815
  33. This study demonstrated that Hypoxia-independent mechanisms of HIF-1alpha expression in astrocytes after ischemic preconditioning. PMID: 28063215
  34. Activation of HIF-1 signaling pathway participates in the Ang II-induced abdominal aortic aneurysm formation in mice PMID: 27497884
  35. Depletion of KDM3A coincided with the loss of SIAH1 induction and the accumulation of dimethylated H3K9 surrounding the SIAH1 promoter. Interestingly, KDM3A expression was also up-regulated by nutritional stress in a HIF-1alpha dependent manner. PMID: 28843785
  36. the proangiogenic capacity of implanted periosteal cells is HIF-1alpha regulated and that metabolic adaptations mediate post-implantation cell survival. PMID: 27058876
  37. Oxidative stress increases in mouse arteriovenous shunts during early maturation, with increased expression of HIF-1alpha and its target genes NOX-2, HO-1, and VEGF-A. PMID: 28163173
  38. HIF1a transcriptional activity is stimulated by Protein kinase A-dependent phosphorylation PMID: 27245613
  39. Data indicate that the HIF-1alpha/VEGF-A axis is an essential aspect of tumor immunity. PMID: 29136509
  40. PROX1 overexpression in DAB2IP-deficient prostate cancer cells could enhance the accumulation of HIF1alpha protein by inhibiting ubiquitin pathway and then consequently induce an epithelial-mesenchymal transition response. PMID: 27476001
  41. findings suggest that hypoxia, through HIF1A, contributes to the development and progression of pulmonary fibrosis through its regulation of ADORA2B expression on alternatively activated macrophages, cell differentiation, and production of profibrotic mediators PMID: 28701304
  42. Death receptor5 pathway and mitochondrial pathway, which are likely mediated by HIF-1alpha, contribute to hypoxia-induced spermatocyte apoptosis. PMID: 28444885
  43. Mechanical low shear stress activates HIF1alpha at atheroprone regions of arteries via nuclear factor-kappaB and Cezanne. HIF1alpha promotes atherosclerosis initiation at these sites by inducing excessive endothelial cell proliferation and inflammation via the induction of glycolysis enzymes. PMID: 28882872
  44. CRP can upregulate vascular endothelial growth factor-A (VEGF-A) expression by activating hypoxia inducible factor-1alpha (HIF-1alpha) in ADSCs. PMID: 27526687
  45. induction of HIF-1alpha mediated transcriptional up-regulation of pro-apoptotic/inflammatory signaling contributes to astrocyte cell death during thiamine deficiency. PMID: 29045486
  46. Smad3 binding to the -335 hypoxia-responsive element of the COL1A2 promoter required HIF-1alpha both in vitro and in kidney lysate from the disease model, suggesting formation of an HIF-1alpha-Smad3 transcriptional complex. Thus, HIF-1alpha-Smad3 has a novel interaction in glomerulosclerosis. PMID: 27503806
  47. These results suggest that cyclosporine A (CsA) exhibits antifibrotic effects by degrading HIF-1alpha and that the CsA-HIF-1alpha axis provides new insights into therapeutic options for the treatment of pulmonary fibrosis. PMID: 28446589
  48. HIF-1alpha protein levels in the ipsilateral hemisphere were higher in the WT mice than Par-1 KO mice after glioma cell implantation. PMID: 26463974
  49. We conclude that HIF-1 is not a major regulator of Vegfa expression during wound healing; rather, it serves to maintain basal levels of expression of Vegfa and its target genes in intact skin, which are required for optimal granulation tissue formation in response to wounding. PMID: 28686658
  50. hypoxic IL-22 upregulation is dependent on HIF-1alpha PMID: 27534553

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Subcellular Location Cytoplasm. Nucleus. Nucleus speckle.
Tissue Specificity Ubiquitous.
Database Links

KEGG: mmu:15251

STRING: 10090.ENSMUSP00000021530

UniGene: Mm.3879

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