Mouse pancreatic alpha amylase (AMY2A)ELISA kit

Code CSB-EL001689MO
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
pancreatic alpha amylase (AMY2A)
Alternative Names
Amy2 ELISA Kit; Amy2a ELISA Kit; Pancreatic alpha-amylase ELISA Kit; PA ELISA Kit; EC 3.2.1.1 ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase ELISA Kit
Abbreviation
AMY2A
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
9.38 mU/mL-600 mU/mL
Sensitivity
2.3 mU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse AMY2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 92-105
1:2 Average % 92
Range % 88-96
1:4 Average % 86
Range % 80-92
1:8 Average % 89
Range % 84-97
Recovery
The recovery of mouse AMY2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 87-97
EDTA plasma (n=4) 101 94-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected
600 2.680 2.714 2.697 2.563
300 2.164 2.235 2.200 2.066
150 1.467 1.421 1.444 1.310
75 0.759 0.811 0.785 0.651
37.5 0.533 0.564 0.549 0.415
18.75 0.317 0.326 0.322 0.188
9.38 0.227 0.241 0.234 0.100
0 0.128 0.139 0.134  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse AMY2A ELISA Kit was designed for the quantitative measurement of Mouse AMY2A protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.38 mU/mL-600 mU/mL and the sensitivity is 2.3 mU/mL.

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Target Background

Subcellular Location
Secreted, extracellular space.
Protein Families
Glycosyl hydrolase 13 family
Database Links
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