Mouse procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

Code CSB-E12775m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Mouse PⅠNP ELISA Kit was designed for the quantitative measurement of Mouse PⅠNP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.7 pg/mL-300 pg/mL and the sensitivity is 1.17 pg/mL.

Target Name procollagen Ⅰ N-terminal peptide,PⅠNP
Alternative Names procollagen 1 N-terminal peptide,P1NP, procollagen I N-terminal peptide,PINP
Abbreviation PⅠNP
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 4.7 pg/mL-300 pg/mL
Sensitivity 1.17 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PINP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 92  
Range % 87-98  
1:200 Average % 95  
Range % 91-98  
1:400 Average % 91  
Range % 85-96  
1:800 Average % 94  
Range % 88-102  
The recovery of mouse PINP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 89-99  
EDTA plasma (n=4) 89 82-94  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
300 2.413 2.313 2.363 2.261  
150 1.598 1.498 1.548 1.446  
75 0.940 0.890 0.915 0.813  
37.5 0.489 0.479 0.484 0.382  
18.75 0.316 0.306 0.311 0.209  
9.4 0.207 0.205 0.206 0.104  
4.7 0.145 0.142 0.144 0.042  
0 0.103 0.101 0.102    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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