Mouse procollagen type III (HPCⅢ) ELISA Kit

Code CSB-E17125m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
procollagen type III (HPCⅢ)
Alternative Names
procollagen type 3 (HPC3) ,procollagen type III (HPCⅢ)
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
3.9 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:100 Average % 97    
Range % 92-101    
1:200 Average % 95    
Range % 90-100    
1:400 Average % 91    
Range % 85-95    
1:800 Average % 102    
Range % 98-107    
The recovery of mouse HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
Sample Type Average % Recovery Range    
Serum (n=5) 89 85-93    
EDTA plasma (n=4) 84 80-88    
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
pg/ml OD1 OD2 Average Corrected    
1000 2.492 2.343 2.418 2.316    
500 1.825 1.716 1.771 1.669    
250 0.947 1.004 0.976 0.874    
125 0.571 0.582 0.577 0.475    
62.5 0.359 0.339 0.349 0.247    
31.2 0.246 0.237 0.242 0.140    
15.6 0.164 0.171 0.168 0.066    
0 0.098 0.105 0.102      
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Mouse HPCⅢ ELISA Kit was designed for the quantitative measurement of Mouse HPCⅢ protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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