Pig Chitinase-3-like protein 1(CHI3L1) ELISA kit

Instructions
Code CSB-EL005346PI
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chitinase 3-like 1 (cartilage glycoprotein-39)
Alternative Names CHI3L1 ELISA Kit; Chitinase-3-like protein 1 ELISA Kit; 38 kDa heparin-binding glycoprotein ELISA Kit; Signal-processing protein ELISA Kit; gp38k ELISA Kit
Abbreviation CHI3L1
Uniprot No. Q29411
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 ng/mL-800 ng/mL
Sensitivity 3.12 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig CHI3L1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 82-92
1:2 Average % 96
Range % 90-102
1:4 Average % 89
Range % 84-95
1:8 Average % 91
Range % 87-98
Recovery
The recovery of pig CHI3L1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 100 95-104
EDTA plasma (n=4) 92 88-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
800 2.481 2.352 2.417 2.230
400 1.724 1.647 1.686 1.499
200 0.975 1.031 1.003 0.816
100 0.581 0.569 0.575 0.388
50 0.378 0.365 0.372 0.185
25 0.323 0.337 0.330 0.143
12.5 0.257 0.248 0.253 0.066
0 0.188 0.186 0.187  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung (By similarity). Stimulates migration and adhesion of cultured vascular smooth muscle cells.
Subcellular Location Secreted, extracellular space, Cytoplasm, Cytoplasm, perinuclear region, Endoplasmic reticulum
Protein Families Glycosyl hydrolase 18 family
Tissue Specificity Detected in smooth muscle cells in atherosclerotic plaques. Detected in regions of vascular occlusion in the aorta.
Database Links

KEGG: ssc:396865

STRING: 9823.ENSSSCP00000016408

UniGene: Ssc.90

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