Pig Clusterin(CLU) ELISA kit

Code CSB-EL005595PI
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q29549
Lead Time 7-14 working days
Abbreviation CLU
Target Name clusterin
Alias AAG4, APOJ, CLI, KUB1, MGC24903, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, aging-associated protein 4|apolipoprotein J|clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 29094
RGD 1307222
MGI 1921398
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig CLU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1000 Average % 97
Range % 91-102
1:2000 Average % 93
Range % 87-97
1:4000 Average % 95
Range % 90-100
1:8000 Average % 100
Range % 94-104
Recovery
The recovery of pig CLU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 87-97
EDTA plasma (n=4) 94 88-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
200 0.180 0.176 0.178
100 0.403 0.396 0.400
50 0.662 0.645 0.654
25 0.921 0.884 0.903
12.5 1.377 1.391 1.384
6.25 1.882 1.827 1.855
3.12 2.192 2.100 2.146
0 2.542 2.469 2.506
References
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Function Functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Promotes apoptosis when in the nucleus. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation (By similarity).
Subcellular Location Secreted, Cytoplasmic vesicle, secretory vesicle, chromaffin granule, Nucleus, Cytoplasm, Mitochondrion membrane, Peripheral membrane protein, Cytoplasmic side, Cytoplasm, cytosol, Microsome, Endoplasmic reticulum
Protein Families Clusterin family
Tissue Specificity Highest levels in brain and liver; lower levels are detected in other tissues, including the aorta. Abundant in senescent porcine pituitary colloids.
Database Links

KEGG: ssc:397025

STRING: 9823.ENSSSCP00000010325

UniGene: Ssc.11992

Pathway Complement and coagulation cascades

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