Pig Granulocyte-macrophage colony-stimulating factor(CSF2) ELISA kit

Instructions
Code CSB-EL006045PI
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name colony stimulating factor 2 (granulocyte-macrophage)
Alternative Names CSF2Granulocyte-macrophage colony-stimulating factor ELISA kit; GM-CSF ELISA kit; Colony-stimulating factor ELISA kit; CSF ELISA kit
Abbreviation CSF2
Uniprot No. Q29118
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig CSF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 86-97
1:2 Average % 96
Range % 89-100
1:4 Average % 93
Range % 88-98
1:8 Average % 101
Range % 94-106
Recovery
The recovery of pig CSF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 87-98
EDTA plasma (n=4) 97 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.176 2.146 2.161 1.980
500 1.949 1.863 1.906 1.725
250 1.383 1.335 1.359 1.178
125 0.801 0.766 0.784 0.603
62.5 0.498 0.484 0.491 0.310
31.2 0.329 0.318 0.324 0.143
15.6 0.245 0.240 0.243 0.062
0 0.183 0.179 0.181  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Gene References into Functions
  1. CSF2 stimulates proliferation of trophectoderm cells by activation of the PI3K-and ERK1/2 MAPK-dependent MTOR signal transduction cascades. PMID: 24520418
Subcellular Location Secreted
Protein Families GM-CSF family
Database Links

KEGG: ssc:397208

STRING: 9823.ENSSSCP00000020413

UniGene: Ssc.382

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