Pig Heme oxygenase 1(HMOX1) ELISA kit

Instructions
Code CSB-EL010583PI
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name heme oxygenase (decycling) 1
Alternative Names HO-1, HSP32, bK286B10, heme oxygenase (decyclizing) 1
Abbreviation HMOX1
Uniprot No. P32394
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %92-98
1:2Average %103
Range %99-103
1:4Average %93
Range %89-99
1:8Average %92
Range %87-98
Recovery
The recovery of pig MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9591-98
EDTA plasma (n=4)9086-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.422 2.546 2.484 2.393
251.998 1.997 1.998 1.907
12.51.560 1.644 1.602 1.511
6.251.160 1.259 1.210 1.119
3.120.795 0.764 0.780 0.689
1.560.355 0.357 0.356 0.265
0.780.215 0.214 0.215 0.124
00.094 0.088 0.091
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Subcellular Location Microsome, Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side
Protein Families Heme oxygenase family
Database Links

KEGG: ssc:445512

UniGene: Ssc.115

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