Pig Hormone-sensitive lipase(LIPE) ELISA kit

Instructions
Code CSB-EL012975PI
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lipase, hormone-sensitive
Alternative Names LIPE ELISA Kit; Hormone-sensitive lipase ELISA Kit; HSL ELISA Kit; EC 3.1.1.79 ELISA Kit
Abbreviation LIPE
Uniprot No. Q68J42
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.39 U/mL-25 U/mL
Sensitivity 0.09 U/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig LIPE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %86-95
1:2Average %102
Range %97-107
1:4Average %91
Range %85-97
1:8Average %97
Range %91-103
Recovery
The recovery of pig LIPE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)9790-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/mlOD1OD2AverageCorrected
252.074 2.198 2.136 1.959
12.51.358 1.369 1.364 1.187
6.250.830 0.901 0.866 0.689
3.120.561 0.572 0.567 0.390
1.560.375 0.396 0.386 0.209
0.780.299 0.285 0.292 0.115
0.390.234 0.246 0.240 0.063
00.178 0.176 0.177
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Subcellular Location Cell membrane, Membrane, caveola, Cytoplasm, cytosol
Protein Families 'GDXG' lipolytic enzyme family
Database Links

KEGG: ssc:397583

STRING: 9823.ENSSSCP00000003269

UniGene: Ssc.6784

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