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| Code | CSB-E06782p |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 80-100 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-110 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 85-105 | |||||
| The recovery of pig IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 83-104 | ||||
| EDTA plasma (n=4) | 98 | 90-108 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Pig IL1B ELISA Kit was designed for the quantitative measurement of Pig IL1B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.9 pg/mL-250 pg/mL and the sensitivity is 0.975 pg/mL .
Sample type: Cell culture supernatant
Sample species: Pig
Sample dilution: No dilution
Review: The results are very satisfactory. I used the cell culture medium supernatant, and the other kits had interfering factors, but the CUSABIO did not, and after trying several other kits, I found that this brand was the only one that worked well.
By Anonymous
Hello, please advise if the Pig IL-1b ELISA kit (#CSB-E06782p) recognizes only mature IL-1b or both mature and pro-IL-1b protein. Thank you.
