Pig apolipoprotein E (Apo-E) ELISA Kit

Code CSB-E17889p
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P18650
Lead Time 3-5 working days
Abbreviation APOE
Protein Biological Process 1 Apolipoprotein
Target Name apolipoprotein E (Apo-E)
Alias AD2, LDLCQ5, LPG, MGC1571, apolipoprotein E3
Species Sus scrofa (Pig)
Protein Biological Process 3 Lipid transport
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 ng/mL-2000 ng/mL
Sensitivity 7.81 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
HGNC 613
RGD 2138
MGI 88057
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 96
Range % 85-105
1:200 Average % 90
Range % 89-98
1:400 Average % 96
Range % 82-103
1:800 Average % 97
Range % 92-99
Recovery
The recovery of pig Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 92-105
EDTA plasma (n=4) 95 85-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average  
2000 0.131 0.134 0.133  
1000 0.223 0.226 0.225  
500 0.335 0.341 0.338  
250 0.562 0.553 0.558  
125 0.862 0.860 0.861  
62.5 1.192 1.214 1.203  
31.25 1.589 1.607 1.598  
0 2.417 2.395 2.406  
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Function Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
Subcellular Location Secreted
Protein Families Apolipoprotein A1/A4/E family
Tissue Specificity Secreted in plasma.
Database Links

KEGG: ssc:397576

STRING: 9823.ENSSSCP00000003343

UniGene: Ssc.1342

Pathway Cholesterol metabolism

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