Pig coagulation factor Ⅶ,FⅦ ELISA Kit

Instructions
Code CSB-E15001p
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name coagulation factor XII (Hageman factor)
Alternative Names F12Coagulation factor XII ELISA kit; EC 3.4.21.38 ELISA kit; Hageman factor ELISA kit; HAF) [Cleaved into: Coagulation factor XIIa heavy chain; Coagulation factor XIIa light chain] ELISA kit
Abbreviation F12
Uniprot No. O97507
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 μg/mL-40 μg/mL
Sensitivity 0.156 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Blood Coagulation
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig FⅦ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 81-91
1:2 Average % 104
Range % 100-108
1:4 Average % 96
Range % 93-99
1:8 Average % 87
Range % 83-90
Recovery
The recovery of pig FⅦ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 105 101-109
EDTA plasma (n=4) 87 83-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average Corrected
40 2.350 2.204 2.277 2.159
20 2.101 2.000 2.051 1.933
10 1.661 1.720 1.691 1.573
5 1.135 1.201 1.168 1.050
2.5 0.634 0.602 0.618 0.500
1.25 0.443 0.415 0.429 0.311
0.625 0.258 0.261 0.260 0.142
0 0.119 0.117 0.118  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa (By similarity).
Subcellular Location Secreted
Protein Families Peptidase S1 family
Database Links

KEGG: ssc:397474

STRING: 9823.ENSSSCP00000024170

UniGene: Ssc.749

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