Pig ferritin,FE ELISA Kit

Code CSB-E13479p
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation FE
Target Name ferritin,FE
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig FE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 103
Range % 100-107
1:2 Average % 91
Range % 87-98
1:4 Average % 96
Range % 90-103
1:8 Average % 88
Range % 85-92
Recovery
The recovery of pig FE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 98-105
EDTA plasma (n=4) 96 91-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
400 2.455 2.545 2.500 2.333
200 2.034 2.030 2.032 1.865
100 1.454 1.432 1.443 1.276
50 0.777 0.744 0.761 0.594
25 0.453 0.423 0.438 0.271
12.5 0.272 0.277 0.275 0.108
6.25 0.212 0.228 0.220 0.053
0 0.173 0.160 0.167  
References
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