Pig growth hormone receptor(GHR) ELISA Kit

Code CSB-E13829p
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P19756
Lead Time 3-5 working days
Abbreviation GHR
Protein Biological Process 1 Immunity
Target Name growth hormone receptor
Alias GHBP, growth hormone binding protein|serum binding protein|somatotropin receptor
Species Sus scrofa (Pig)
Protein Biological Process 3 Endocytosis
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
HGNC 4263
RGD 2687
MGI 95708
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig GHR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 101    
Range % 95-106    
1:2 Average % 100    
Range % 92-105    
1:4 Average % 96    
Range % 86-102    
1:8 Average % 99    
Range % 93-103    
Recovery
The recovery of pig GHR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 96 89-102    
EDTA plasma (n=4) 97 91-100    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
400 1.927 1.968 1.948 1.839    
200 1.521 1.463 1.492 1.383    
100 1.123 1.056 1.090 0.981    
50 0.798 0.787 0.793 0.684    
25 0.468 0.451 0.460 0.351    
12.5 0.296 0.289 0.293 0.184    
6.25 0.199 0.192 0.196 0.087    
0 0.111 0.107 0.109      
Still Have Questions? Leave a Message or Start an on-line Chat
Function Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to, and activates the JAK2/STAT5 pathway.; FUNCTION
Subcellular Location Cell membrane, Single-pass type I membrane protein, Note=On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway, SUBCELLULAR LOCATION: Growth hormone-binding protein: Secreted
Protein Families Type I cytokine receptor family, Type 1 subfamily
Database Links

STRING: 9823.ENSSSCP00000017873

UniGene: Ssc.93799

Pathway Jak-STAT signaling pathway
PI3K-Akt signaling pathway

Related Products

GHR Antibodies

GHR Antibodies for Sus scrofa (Pig)

GHR Proteins

GHR Proteins for Sus scrofa (Pig)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.